Effect of Piper cubeba total extract and isolated lignans on head and neck cancer cell lines and normal fibroblasts.

Autor:	Gusson-Zanetoni JP; São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Science (IBILCE), Campus São José do Rio Preto, Department of Biology, SP, Brazil., da Silva JSGM; São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Science (IBILCE), Campus São José do Rio Preto, Department of Biology, SP, Brazil., Picão TB; São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Science (IBILCE), Campus São José do Rio Preto, Department of Biology, SP, Brazil., Cardin LT; São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Science (IBILCE), Campus São José do Rio Preto, Department of Biology, SP, Brazil., Prates J; São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Science (IBILCE), Campus São José do Rio Preto, Department of Biology, SP, Brazil., Sousa SO; São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Science (IBILCE), Campus São José do Rio Preto, Department of Biology, SP, Brazil., Henrique T; School of Medicine of São José do Rio Preto (FAMERP), Department of Molecular Biology, São José do Rio Preto, Brazil., Oliani SM; São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Science (IBILCE), Campus São José do Rio Preto, Department of Biology, SP, Brazil., Tajara EH; School of Medicine of São José do Rio Preto (FAMERP), Department of Molecular Biology, São José do Rio Preto, Brazil; Department of Genetics and Evolutive Biology, Institute of Biosciences, University of São Paulo, São Paulo, SP, Brazil., E Silva MLA; University of Franca (UNIFRAN), Nucleus of Research in Exact and Technological Sciences, Franca, SP, Brazil., Cunha NL; University of Franca (UNIFRAN), Nucleus of Research in Exact and Technological Sciences, Franca, SP, Brazil., da Silva Gomes AC; São Paulo State University (UNESP), School of Engineering (FEIS), Campus Ilha Solteira, Department of Physical Chemistry, Brazil., Laurentiz RS; São Paulo State University (UNESP), School of Engineering (FEIS), Campus Ilha Solteira, Department of Physical Chemistry, Brazil., Rodrigues-Lisoni FC; São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Science (IBILCE), Campus São José do Rio Preto, Department of Biology, SP, Brazil; São Paulo State University (UNESP), School of Engineering (FEIS), Campus Ilha Solteira, Department of Biology and Animal Science, Brazil. Electronic address: flavia.lisoni@unesp.br.
Jazyk:	angličtina
Zdroj:	Journal of pharmacological sciences [J Pharmacol Sci] 2022 Jan; Vol. 148 (1), pp. 93-102. Date of Electronic Publication: 2021 Oct 07.
DOI:	10.1016/j.jphs.2021.09.002
Abstrakt:	The objective of the present study was to evaluate the action of the crude hydroalcoholic extract of Piper cubeba fruits and isolated lignans (cubebin, dihydrocubebin, ethylcubebin, hinokinin and methylcubebin) on head and neck cancer cells. We evaluated the influence of the Piper cubeba extract and isolated lignans (10, 50 e 100 μg/mL) for 4, 24, 48 and 72 h, in the larynx (Hep-2) and oral (SCC-25) squamous cell carcinoma cells and normal fibroblasts, on morphology, cell proliferation and migration, cytotoxicity, genotoxicity and gene and protein expression (PTGS2, PTGER3, PTGER4, MMP2, MMP9). The results showed that the P. cubeba extract and different lignans do not alter the cellular morphology, but decrease cell proliferation and migration, have low cytotoxic and genotoxic effects, probably due to the alteration of the expression of genes and proteins involved with inflammatory process. From these data, we can conclude that the lignans cubebin and methylcubebin had a greater effect on head and neck cancer cells in the antiproliferative, antimigratory and genotoxic action, and could be the target of the development of new therapies including possible new drugs as a therapeutic resource for the treatment of head and neck cancer due to its immense range of biological properties. Competing Interests: Declaration of competing interest The authors declared that they have no conflict of interest. (Copyright © 2021 The Authors. Production and hosting by Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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