Comparison of novel rapid diagnostic of blood culture identification and antimicrobial susceptibility testing by Accelerate Pheno system and BioFire FilmArray Blood Culture Identification and BioFire FilmArray Blood Culture Identification 2 panels.

Autor: Sze DTT; Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong, Special Administrative Region, China., Lau CCY; Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong, Special Administrative Region, China., Chan TM; Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong, Special Administrative Region, China., Ma ESK; Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong, Special Administrative Region, China., Tang BSF; Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong, Special Administrative Region, China. bonetang@hksh.com.; Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong, Special Administrative Region, China. bonetang@hksh.com.
Jazyk: angličtina
Zdroj: BMC microbiology [BMC Microbiol] 2021 Dec 18; Vol. 21 (1), pp. 350. Date of Electronic Publication: 2021 Dec 18.
DOI: 10.1186/s12866-021-02403-y
Abstrakt: Background: Conventional turnaround time (TAT) for positive blood culture (PBC) identification (ID) and antimicrobial susceptibility testing (AST) is 2-3 days. We evaluated the TAT and ID/AST performance using clinical and seeded samples directly from PBC bottles with different commercial approaches: (1) Accelerate Pheno® system (Pheno) for ID/AST; (2) BioFire® FilmArray® Blood Culture Identification (BCID) Panel and/ or BCID2 for ID; (3) direct AST by VITEK® 2 (direct AST); and (4) overnight culture using VITEK® 2 colony AST.
Results: A total of 141 PBC samples were included in this evaluation. Using MALDI-TOF (Bruker MALDI Biotyper) as the reference method for ID, the overall monomicrobial ID sensitivity/specificity are as follows: Pheno 97.9/99.9%; BCID 100/100%; and BCID2 100/100%, respectively. For AST performance, broth microdilution (BMD) was used as the reference method. For gram-negatives, overall categorical and essential agreements (CA/EA) for each method were: Pheno 90.3/93.2%; direct AST 92.6/88.5%; colony AST 94.4/89.5%, respectively. For gram-positives, the overall CA/EAs were as follows: Pheno 97.2/98.89%; direct AST 97.2/100%; colony AST 97.2/100%, respectively. The BCID/BCID2 and direct AST TATs were around 9-20 h (1/9-19 h for ID with resistance markers/AST), with 15 min/sample hands-on time. In comparison, Pheno TATs were around 8-10 h (1.5/7 h for ID/AST) with 2 min/sample hands-on time, maintains a clinically relevant fast report of antibiotic minimal inhibitory concentration (MIC) and allows for less TAT and hands-on time.
Conclusion: In conclusion, to the best of our knowledge, this is the first study conducted as such in Asia; all studied approaches achieved satisfactory performance, factors such as TAT, panel of antibiotics choices and hands-on time should be considered for the selection of appropriate rapid ID and AST of PBC methods in different laboratory settings.
(© 2021. The Author(s).)
Databáze: MEDLINE
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