Evaluation of the detection of staA, viaB and sopE genes in Salmonella spp. using the polymerase chain reaction (PCR).

Autor: Kariuki F; Department of Biochemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya. franciskariuki@gmail.com., Getanda P; Department of Biochemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya., Nyachieo A; Department of Biochemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya.; Molecular Biology Unit, Institute of Primate Research, P.O. Box 24481-00502, Nairobi, Kenya., Juma G; Department of Biochemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya., Kinyanjui P; Department of Biochemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya., Kamau J; Department of Biochemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya.; Molecular Biology Unit, Institute of Primate Research, P.O. Box 24481-00502, Nairobi, Kenya.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2021 Dec 18; Vol. 204 (1), pp. 25. Date of Electronic Publication: 2021 Dec 18.
DOI: 10.1007/s00203-021-02654-3
Abstrakt: Typhoid fever is caused by the bacteria Salmonella enterica subspecies enterica serovar Typhi (S. Typhi) and remains a significant health problem in many developing countries. Lack of adequate diagnostic capabilities has contributed greatly in making typhoid fever endemic in these regions. Reliable and inexpensive diagnostic tests are needed to improve the management of this disease burden. We evaluated the ability of staA, viaB and sopE genes to detect and differentiate between the three most prevalent Salmonella spp. in Kenya (S. Typhi, S. Typhimurium and S. Enteritidis) using conventional polymerase chain reaction (PCR). The staA primers and viaB primers were found to be specific only for the different strains of S. Typhi, producing PCR products of 585 bp and 540 bp, respectively. The sopE primers was demonstrated to be specific for all Salmonella spp. producing a 465 bp PCR product with no amplification with E. coli and S. boydii bacterial strains.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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