A method for campus-wide SARS-CoV-2 surveillance at a large public university.

Autor: Chang T; Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America.; Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Center for Molecular Biology of RNA, University of California Santa Cruz, Santa Cruz, California, United States of America., Draper JM; Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Center for Molecular Biology of RNA, University of California Santa Cruz, Santa Cruz, California, United States of America.; Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America., Van den Bout A; Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Center for Molecular Biology of RNA, University of California Santa Cruz, Santa Cruz, California, United States of America.; Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America., Kephart E; Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Center for Molecular Biology of RNA, University of California Santa Cruz, Santa Cruz, California, United States of America.; Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America., Maul-Newby H; Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Center for Molecular Biology of RNA, University of California Santa Cruz, Santa Cruz, California, United States of America.; Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America., Vasquez Y; Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Center for Molecular Biology of RNA, University of California Santa Cruz, Santa Cruz, California, United States of America.; Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America., Woodbury J; Thirdwave Analytics, San Francisco, California, United States of America., Randi S; Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America., Pedersen M; Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America., Nave M; Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America., La S; Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America., Gallagher N; Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America., McCabe MM; Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America.; Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America., Dhillon N; Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America.; Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America., Bjork I; Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America., Luttrell M; Business and Accounting Services, University of California Santa Cruz, Santa Cruz, California, United States of America., Dang F; University of California Davis, Davis, California, United States of America., MacMillan JB; Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America.; Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, California, United States of America., Green R; University of California Davis, Davis, California, United States of America., Miller E; Student Health Services, University of California Santa Cruz, Santa Cruz, California, United States of America., Kilpatrick AM; Department of Ecology and Evolutionary Biology, University of California Santa Cruz, Santa Cruz, California, United States of America., Vaske O; Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America.; Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Center for Molecular Biology of RNA, University of California Santa Cruz, Santa Cruz, California, United States of America.; Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America., Stone MD; Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America.; Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Center for Molecular Biology of RNA, University of California Santa Cruz, Santa Cruz, California, United States of America., Sanford JR; Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Center for Molecular Biology of RNA, University of California Santa Cruz, Santa Cruz, California, United States of America.; Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2021 Dec 17; Vol. 16 (12), pp. e0261230. Date of Electronic Publication: 2021 Dec 17 (Print Publication: 2021).
DOI: 10.1371/journal.pone.0261230
Abstrakt: The systematic screening of asymptomatic and pre-symptomatic individuals is a powerful tool for controlling community transmission of infectious disease on college campuses. Faced with a paucity of testing in the beginning of the COVID-19 pandemic, many universities developed molecular diagnostic laboratories focused on SARS-CoV-2 diagnostic testing on campus and in their broader communities. We established the UC Santa Cruz Molecular Diagnostic Lab in early April 2020 and began testing clinical samples just five weeks later. Using a clinically-validated laboratory developed test (LDT) that avoided supply chain constraints, an automated sample pooling and processing workflow, and a custom laboratory information management system (LIMS), we expanded testing from a handful of clinical samples per day to thousands per day with the testing capacity to screen our entire campus population twice per week. In this report we describe the technical, logistical, and regulatory processes that enabled our pop-up lab to scale testing and reporting capacity to thousands of tests per day.
Competing Interests: MDS and JRS are paid consultants and have an ownership interest in SummerBio, a commercial COVID testing laboratory that specializes in institutional surveillance. JW is an employee of Thirdwave Analytics. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
Databáze: MEDLINE
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