Laboratory Diagnostic Capacity for Fungal Infections in Nigerian Tertiary Hospitals: A Gap Analysis Survey.

Autor:	Osaigbovo II; Department of Medical Microbiology, School of Medicine, University of Benin, Benin City, Nigeria.; Department of Medical Microbiology, University of Benin Teaching Hospital, Benin City, Nigeria.; Medical Mycology Society of Nigeria (MMSN), Lagos, Nigeria., Oladele RO; Medical Mycology Society of Nigeria (MMSN), Lagos, Nigeria.; Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Lagos, Nigeria., Orefuwa E; Global Action Fund for Fungal Infections (GAFFI), Geneva, Switzerland., Akanbi OA; Public Health Laboratory Services, Nigeria Centre for Disease Control, Abuja, Federal Capital Territory, Nigeria., Ihekweazu C; Office of the Director General, Nigeria Centre for Disease Control, Abuja, Federal Capital Territory, Nigeria.
Jazyk:	angličtina
Zdroj:	West African journal of medicine [West Afr J Med] 2021 Nov 30; Vol. Vol. 38 (11), pp. 1065-1071.
Abstrakt:	Background: An estimated 11.8% of Nigerians suffer from invasive fungal infections (IFIs) yearly. Laboratory capacity to diagnose IFIs in Nigeria has not been objectively assessed. Objective: To identify the gaps in laboratory capacity for diagnosis of IFIs in Nigerian tertiary hospitals. Methods: Clinical microbiologists in Nigerian tertiary hospitals were invited to partake in a 21-item online survey via a professional chat group and email. A descriptive crosssectional study of survey responses was conducted. Frequencies were computed for microscopy, culture, antifungal sensitivity, and non-culture based diagnostic modalities. Findings: Respondents were from 22 tertiary hospitals spread across the six geo-political zones of Nigeria. Gaps identified include absence of mycology laboratory/bench in 5/22 (22.7%), no access to a biosafety cabinet in 5/22 (22.7%), lack of laboratory scientists formally trained in mycology in 9/22 (40.9%), lack of participation in external quality assurance in all (100%), lack of automated blood culture facilities in 9/22 (40.9%), no yeast identification beyond germ tube test in12/22 (54.5%), and no anti-fungal sensitivity testing in 17/22 (77.3%). Galactomannan, cryptococcal antigen lateral flow assay and latex agglutination tests are used in 1(4.5%), 3 (13.6%) and 5 (22.7%) centres respectively; antigen/antibody based non-culture diagnostics were totally absent in 12/22 (54.5%) hospitals. Conclusion: Nigerian tertiary hospitals have gaps in the laboratory capacity to diagnose invasive fungal infections despite the significant size of the population at risk of these life-threatening infections in the country. Economically feasible diagnostic solutions and models as well as capacity building are urgently required. Competing Interests: The Authors declare that no competing interest exists (Copyright © 2021 by West African Journal of Medicine.)
Databáze:	MEDLINE
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