A SARS-CoV-2 ferritin nanoparticle vaccine elicits protective immune responses in nonhuman primates.

Autor: Joyce MG; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA., King HAD; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; U.S. Military HIV Research Program, WRAIR, Silver Spring, MD 20910, USA., Elakhal-Naouar I; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; Diagnostics and Countermeasures Branch, WRAIR, Silver Spring, MD 20910, USA., Ahmed A; Viral Diseases Branch, WRAIR, Silver Spring, MD 20910, USA., Peachman KK; Diagnostics and Countermeasures Branch, WRAIR, Silver Spring, MD 20910, USA., Macedo Cincotta C; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; Diagnostics and Countermeasures Branch, WRAIR, Silver Spring, MD 20910, USA., Subra C; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; U.S. Military HIV Research Program, WRAIR, Silver Spring, MD 20910, USA., Chen RE; Department of Medicine, Washington University, St. Louis, MO 63130, USA.; Department of Pathology and Immunology, Washington University, St. Louis, MO 63130, USA., Thomas PV; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA., Chen WH; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA., Sankhala RS; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA., Hajduczki A; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA., Martinez EJ; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA., Peterson CE; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA., Chang WC; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA., Choe M; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA., Smith C; Center for Molecular Microscopy, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA., Lee PJ; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA., Headley JA; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA., Taddese MG; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA., Elyard HA; Bioqual, Rockville, MD 20850, USA., Cook A; Bioqual, Rockville, MD 20850, USA., Anderson A; U.S. Military HIV Research Program, WRAIR, Silver Spring, MD 20910, USA.; Oak Ridge Institute of Science and Education, Oak Ridge, TN 37830, USA., McGuckin Wuertz K; U.S. Military HIV Research Program, WRAIR, Silver Spring, MD 20910, USA., Dong M; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; U.S. Military HIV Research Program, WRAIR, Silver Spring, MD 20910, USA., Swafford I; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; U.S. Military HIV Research Program, WRAIR, Silver Spring, MD 20910, USA., Case JB; Department of Medicine, Washington University, St. Louis, MO 63130, USA., Currier JR; Viral Diseases Branch, WRAIR, Silver Spring, MD 20910, USA., Lal KG; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; U.S. Military HIV Research Program, WRAIR, Silver Spring, MD 20910, USA., Molnar S; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; U.S. Military HIV Research Program, WRAIR, Silver Spring, MD 20910, USA., Nair MS; Diamond AIDS Research Center, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA., Dussupt V; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; U.S. Military HIV Research Program, WRAIR, Silver Spring, MD 20910, USA., Daye SP; Center for Infectious Diseases Research, WRAIR, Silver Spring, MD 20910, USA., Zeng X; Division of Pathology, United States Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA., Barkei EK; Veterinary Pathology Department, WRAIR, Silver Spring, MD 20910, USA., Staples HM; Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio, TX 78227, USA., Alfson K; Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio, TX 78227, USA., Carrion R; Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio, TX 78227, USA., Krebs SJ; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; U.S. Military HIV Research Program, WRAIR, Silver Spring, MD 20910, USA., Paquin-Proulx D; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; U.S. Military HIV Research Program, WRAIR, Silver Spring, MD 20910, USA., Karasavva N; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; Diagnostics and Countermeasures Branch, WRAIR, Silver Spring, MD 20910, USA., Polonis VR; U.S. Military HIV Research Program, WRAIR, Silver Spring, MD 20910, USA., Jagodzinski LL; Diagnostics and Countermeasures Branch, WRAIR, Silver Spring, MD 20910, USA., Amare MF; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA., Vasan S; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; U.S. Military HIV Research Program, WRAIR, Silver Spring, MD 20910, USA., Scott PT; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA., Huang Y; Diamond AIDS Research Center, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA., Ho DD; Diamond AIDS Research Center, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA., de Val N; Center for Molecular Microscopy, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA., Diamond MS; Department of Medicine, Washington University, St. Louis, MO 63130, USA.; Department of Pathology and Immunology, Washington University, St. Louis, MO 63130, USA.; Department of Molecular Microbiology, Washington University, St. Louis, MO 63130, USA., Lewis MG; Bioqual, Rockville, MD 20850, USA., Rao M; U.S. Military HIV Research Program, WRAIR, Silver Spring, MD 20910, USA., Matyas GR; U.S. Military HIV Research Program, WRAIR, Silver Spring, MD 20910, USA., Gromowski GD; Viral Diseases Branch, WRAIR, Silver Spring, MD 20910, USA., Peel SA; Diagnostics and Countermeasures Branch, WRAIR, Silver Spring, MD 20910, USA., Michael NL; Center for Infectious Diseases Research, WRAIR, Silver Spring, MD 20910, USA., Bolton DL; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; U.S. Military HIV Research Program, WRAIR, Silver Spring, MD 20910, USA., Modjarrad K; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA.
Jazyk: angličtina
Zdroj: Science translational medicine [Sci Transl Med] 2022 Feb 16; Vol. 14 (632), pp. eabi5735. Date of Electronic Publication: 2022 Feb 16.
DOI: 10.1126/scitranslmed.abi5735
Abstrakt: The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants stresses the continued need for next-generation vaccines that confer broad protection against coronavirus disease 2019 (COVID-19). We developed and evaluated an adjuvanted SARS-CoV-2 spike ferritin nanoparticle (SpFN) vaccine in nonhuman primates. High-dose (50 μg) SpFN vaccine, given twice 28 days apart, induced a Th1-biased CD4 T cell helper response and elicited neutralizing antibodies against SARS-CoV-2 wild-type and variants of concern, as well as against SARS-CoV-1. These potent humoral and cell-mediated immune responses translated into rapid elimination of replicating virus in the upper and lower airways and lung parenchyma of nonhuman primates following high-dose SARS-CoV-2 respiratory challenge. The immune response elicited by SpFN vaccination and resulting efficacy in nonhuman primates supports the utility of SpFN as a vaccine candidate for SARS-causing betacoronaviruses.
Databáze: MEDLINE