Salivary Gland Development in Culture.
| Autor: | Gaete M; Department of Anatomy, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile. mgaets@uc.cl., Teshima THN; Department of Oral Medicine, Eastman Dental Institute, University College London, London, UK., Chatzeli L; Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK., Tucker AS; Centre for Craniofacial and Regenerative Biology, King's College London, London, UK. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2403, pp. 277-294. |
| DOI: | 10.1007/978-1-0716-1847-9_19 |
| Abstrakt: | Salivary glands are branching organs which develop by bud and cleft formation to create an organ with a large surface area. The epithelium and mesenchyme signal back and forth to control this branching process, with additional cues provided by the parasympathetic nerves and blood vessels that surround the developing branches. This branching morphogenesis can be recapitulated successfully in organ culture , allowing access to the tissue to follow development and manipulate the tissue interactions, and signals. To culture glands, the filter-grid method has been widely used, allowing the development of salivary glands cultured as a whole organ, or the gland epithelium in isolation, or with the surrounding craniofacial tissue in a cranial slice. Here, we describe the methods for each approach and show the applicability of culturing glands from a wide variety of species: mouse , snake, and human. The resulting samples and data from these cultures can be employed for morphological and molecular analysis, with some examples described in this chapter, bringing valuable knowledge to our understanding of branching morphogenesis. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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