Single-Conidium Encapsulation in Oil-in-Water Pickering Emulsions at High Encapsulation Yield.

Autor: Kotliarevski L; Department of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization (ARO), Volcani Institute, Rishon Lezion, Israel.; Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel., Mani KA; Department of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization (ARO), Volcani Institute, Rishon Lezion, Israel.; Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel., Feldbaum RA; Department of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization (ARO), Volcani Institute, Rishon Lezion, Israel., Yaakov N; Department of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization (ARO), Volcani Institute, Rishon Lezion, Israel., Belausov E; Department of Ornamental Plants and Agricultural Biotechnology, Institute of Plant Science, Agricultural Research Organization (ARO), Volcani Institute, Rishon Lezion, Israel., Zelinger E; Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel., Ment D; Department of Plant Pathology and Weed Research, Institute of Plant Protection, Agricultural Research Organization (ARO), Volcani Institute, Rishon Lezion, Israel., Mechrez G; Department of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization (ARO), Volcani Institute, Rishon Lezion, Israel.
Jazyk: angličtina
Zdroj: Frontiers in chemistry [Front Chem] 2021 Nov 29; Vol. 9, pp. 726874. Date of Electronic Publication: 2021 Nov 29 (Print Publication: 2021).
DOI: 10.3389/fchem.2021.726874
Abstrakt: This study presents an individual encapsulation of fungal conidia in an oil-in-water Pickering emulsion at a single-conidium encapsulation yield of 44%. The single-conidium encapsulation yield was characterized by analysis of confocal microscopy micrographs. Mineral oil-in-water emulsions stabilized by amine-functionalized titania dioxide (TiO 2 -NH 2 or titania-NH 2 ) particles were prepared. The structure and the stability of the emulsions were investigated at different compositions by confocal microscopy and a LUMiSizer ® respectively. The most stable emulsions with a droplet size suitable for single-conidium encapsulation were further studied for their individual encapsulation capabilities. The yields of individual encapsulation in the emulsions; i.e., the number of conidia that were individually encapsulated out of the total number of conidia, were characterized by confocal microscopy assay. This rapid, easy to use approach to single-conidium encapsulation, which generates a significantly high yield with eco-friendly titania-based emulsions, only requires commonly used emulsification and agitation methods.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2021 Kotliarevski, Mani, Feldbaum, Yaakov, Belausov, Zelinger, Ment and Mechrez.)
Databáze: MEDLINE
Externí odkaz: