Quantification of torque teno virus (TTV) DNA in saliva and plasma samples in patients at short time before and after kidney transplantation.

Autor: Batista AM; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, University of São Paulo School of Medicine, São Paulo, Brazil., Caetano MW; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, University of São Paulo School of Medicine, São Paulo, Brazil.; Department of Stomatology, University of São Paulo School of Dentistry, São Paulo, Brazil., Stincarelli MA; Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy., Mamana AC; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, University of São Paulo School of Medicine, São Paulo, Brazil., Zerbinati RM; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, University of São Paulo School of Medicine, São Paulo, Brazil., Sarmento DJS; Department of Stomatology, University of São Paulo School of Dentistry, São Paulo, Brazil.; Department of Oral Medicine, State University of Paraiba, Araruna, Brazil., Gallottini M; Department of Stomatology, University of São Paulo School of Dentistry, São Paulo, Brazil., Caixeta RAV; Department of Stomatology, University of São Paulo School of Dentistry, São Paulo, Brazil., Medina-Pestana J; Division of Renal Transplantation, Kidney and Hypertension Hospital, Federal University of São Paulo School of Medicine, São Paulo, Brazil., Hasséus B; Department of Oral Medicine and Pathology, University of Gothenburg Institute of Odontology, Gothenburg, Sweden., Zanella L; Laboratory of Integrative Biology (LIBi), Scientific and Technological Bioresource Nucleus - Center for Excellence in Translational Medicine (BIOREN - CEMT), Universidad de La Frontera, Temuco, Chile., Tozetto-Mendoza TR; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, University of São Paulo School of Medicine, São Paulo, Brazil., Giannecchini S; Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy., Braz-Silva PH; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, University of São Paulo School of Medicine, São Paulo, Brazil.; Department of Stomatology, University of São Paulo School of Dentistry, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Journal of oral microbiology [J Oral Microbiol] 2021 Dec 12; Vol. 14 (1), pp. 2008140. Date of Electronic Publication: 2021 Dec 12 (Print Publication: 2022).
DOI: 10.1080/20002297.2021.2008140
Abstrakt: Background: Several reports have proposed that the viral load of torque teno virus (TTV) in plasma is a biomarker of immune function in solid organ transplantation (SOT) and in allogeneic hematopoietic stem cell transplantation. Additionally, for the latter one, TTV-DNA quantification in saliva has also been suggested.
Aim: to investigate the correlation between the TTV viral load and immune function in paired saliva and plasma samples in patients on kidney transplantation.
Materials and Methods: TTV-DNA viral load was quantified in paired samples of saliva and plasma from 71 patients before and a short-time after renal-transplantation by real-time PCR.
Results: The data obtained from 213 paired samples showed a slight consistency in the comparison between saliva and plasma, with prevalence of TTV-DNA being 58%, 52% and 60% in saliva samples and 60%, 73% and 90% in plasma samples before and at 15-20 and 45-60 days after transplantation, respectively. Additionally, a high TTV viral load was observed in plasma at 15-20 and 45-60 days after transplantation compared to that observed in saliva at the same time.
Conclusions: Overall, monitoring TTV-DNA in saliva samples could be an additional fast non-invasive option to assess the immune functionality in SOT populations.
Competing Interests: No potential conflict of interest was reported by the author(s).
(© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)
Databáze: MEDLINE
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