Propolis in Oral Healthcare: Antibacterial Activity of a Composite Resin Enriched With Brazilian Red Propolis.

Autor:	Oliveira JMDS; Postgraduate Program of Chemistry and Biotechnology, Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió, Brazil.; Postgraduate Program in Health Research, Cesmac University Center, Maceió, Brazil., Cavalcanti TFS; Postgraduate Program in Materials, Center of Technology, Federal University of Alagoas, Maceió, Brazil.; Faculty of Dentistry, Federal University of Alagoas, Maceió, Brazil., Leite IF; Postgraduate Program in Health Research, Cesmac University Center, Maceió, Brazil., Dos Santos DMRC; Postgraduate Program Multicenter of Biochemistry and Molecular Biology, Institute of Pharmaceutical Sciences, Federal University of Alagoas, Maceió, Brazil., Porto ICCM; Faculty of Dentistry, Federal University of Alagoas, Maceió, Brazil.; Postgraduate Program in Pharmaceutical Sciences, Institute of Pharmaceutical Sciences, Federal University of Alagoas, Maceió, Brazil., de Aquino FLT; Postgraduate Program in Health Sciences, Institute of Biological and Health Sciences, Federal University of Alagoas, Maceió, Brazil., Sonsin AF; Postgraduate Program in Physics, Institute of Physics, Federal University of Alagoas, Maceió, Brazil., Lins RML; Faculty of Dentistry, Federal University of Alagoas, Maceió, Brazil., Vitti RP; Faculty of Dentistry, Hermínio Ometto Foundation, Araras, Brazil., de Freitas JD; Department of Chemistry, Federal Institute of Alagoas, Maceió, Brazil., Barreto EO; Postgraduate Program in Health Sciences, Institute of Biological and Health Sciences, Federal University of Alagoas, Maceió, Brazil., de Souza ST; Postgraduate Program in Physics, Institute of Physics, Federal University of Alagoas, Maceió, Brazil., Kamiya RU; Postgraduate Program in Health Sciences, Institute of Biological and Health Sciences, Federal University of Alagoas, Maceió, Brazil., do Nascimento TG; Postgraduate Program in Pharmaceutical Sciences, Institute of Pharmaceutical Sciences, Federal University of Alagoas, Maceió, Brazil., Tonholo J; Postgraduate Program of Chemistry and Biotechnology, Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in pharmacology [Front Pharmacol] 2021 Nov 29; Vol. 12, pp. 787633. Date of Electronic Publication: 2021 Nov 29 (Print Publication: 2021).
DOI:	10.3389/fphar.2021.787633
Abstrakt:	The aim of this study was to obtain a Brazilian red propolis (BRP) enriched composite resin and to perform the characterization of its antibacterial activity, mechanical, and physical-chemical properties. Brazilian red propolis ethyl acetate extract (EABRP) was characterized by LC-ESI-Orbitrap-FTMS, UPLC-DAD, antibacterial activity, total flavonoids content, and radical scavenging capacity. BRP was incorporated to a commercial composite resin (RC) to obtain BRP enriched composite at 0.1, 0.15 and 0.25% (RP10, RP15 and RP25, respectively). The antibacterial activity RPs was evaluated against Streptococcus mutans by contact direct test and expressed by antibacterial ratio. The RPs were characterized as its cytotoxicity against 3T3 fibroblasts, flexural strength (FS), Knoop microhardness (KHN), post-cure depth (CD), degree of conversion (DC%), water sorption (Wsp), water solubility (Wsl), average roughness (Ra), and thermal analysis. Were identified 50 chemical compounds from BRP extract by LC-ESI-Orbitrap-FTMS. EABRP was bacteriostatic and bactericide at 125 and 500 μg/ml, respectively. The RP25 exhibited antibacterial ratio of 90.76% after 1 h of direct contact with S. mutans ( p < 0.0001) while RC no showed significative antibacterial activity ( p = 0.1865), both compared with cell control group. RPs and RC no showed cytotoxicity. RPs exhibited CD from 2.74 to 4.48 mm, DC% from 80.70 to 83.96%, Wsp from 17.15 to 21.67 μg/mm 3 , Wsl from 3.66 to 4.20 μg/mm 3 , Ra from 14.48 to 20.76 nm. RPs showed thermal resistance between 448-455°C. The results support that propolis can be used on development of modified composite resins that show antibacterial activity and that have compatible mechanical and physical-chemical properties to the indicate for composite resins. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Oliveira, Cavalcanti, Leite, dos Santos, Porto, de Aquino, Sonsin, Lins, Vitti, de Freitas, Barreto, de Souza, Kamiya, do Nascimento and Tonholo.)
Databáze:	MEDLINE
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