Antiinflammatory and antiinfective effect of caffeine in a mouse model of disseminated salmonellosis.

Autor: Dorvigny BM; Department of Biology, Federal Rural University of Pernambuco, Recife, Brazil., Tavares LS; Department of Biology, Federal Rural University of Pernambuco, Recife, Brazil., de Almeida IA; Department of Biology, Federal Rural University of Pernambuco, Recife, Brazil., Santana LN; Department of Biology, Federal Rural University of Pernambuco, Recife, Brazil., de Souza Silva E; Department of Biology, Federal Rural University of Pernambuco, Recife, Brazil., de Souza JKU; Department of Biology, Federal Rural University of Pernambuco, Recife, Brazil., Soares AF; Department of Animal Morphology and Physiology, Federal Rural University of Pernambuco, Recife, Brazil., da Silva Júnior VA; Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil., Lima-Filho JV; Department of Biology, Federal Rural University of Pernambuco, Recife, Brazil.
Jazyk: angličtina
Zdroj: Phytotherapy research : PTR [Phytother Res] 2022 Apr; Vol. 36 (4), pp. 1652-1663. Date of Electronic Publication: 2021 Dec 15.
DOI: 10.1002/ptr.7349
Abstrakt: Caffeine has been reported for its antiinflammatory properties by stimulating phagocytosis. In this study, we investigated the antiinflammatory and antiinfective potential of caffeine in murine macrophage cell cultures and Swiss mice infected with virulent Salmonella enterica serotype typhimurium. Peritoneal macrophages (pMØ) were treated with caffeine on 96-well plates for 24 hr and then infected with Salmonella for 4 hr. In another experiment, the pMØ were first infected with the bacterium for 4 hr and then treated with caffeine for 24 hr. In addition, Swiss mice were inoculated, intraperitoneally, with S. typhimurium and then received caffeine intravenously. Control groups received phosphate-buffered saline (PBS) or dexamethasone. We found that treatments with caffeine increased the macrophage cell viability and reduced the intracellular bacterial load. The administration of caffeine to Swiss mice reduced the infiltration of leukocytes into the peritoneal cavity after the bacterial challenge. Furthermore, the bacterial burdens in the peritoneal fluid, bloodstream, spleen, and liver were decreased by caffeine treatment. The expression levels of tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), IL-6, and inducible nitric oxide synthase (iNOs) were down-regulated after infection in caffeine-treated mice. We can conclude that caffeine has both antiinflammatory and antiinfective properties that can be useful for management of bacterial infections along with antibiotics.
(© 2021 John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz: