ECM-integrin signalling instructs cellular position sensing to pattern the early mouse embryo.

Autor:	Kim EJY; European Molecular Biology Laboratory (EMBL), Heidelberg 69117, Germany.; Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of Biosciences, Universität Heidelberg, Heidelberg 69117, Germany., Sorokin L; Institute of Physiological Chemistry and Pathobiochemistry and Cells in Motion Interfaculty Centre (CiMIC), University of Muenster, Muenster 48149, Germany., Hiiragi T; European Molecular Biology Laboratory (EMBL), Heidelberg 69117, Germany.; Institute for the Advanced Study of Human Biology (WPI-ASHBi), Kyoto University, Kyoto 606-8303, Japan.
Jazyk:	angličtina
Zdroj:	Development (Cambridge, England) [Development] 2022 Jan 01; Vol. 149 (1). Date of Electronic Publication: 2022 Jan 13.
DOI:	10.1242/dev.200140
Abstrakt:	Development entails patterned emergence of diverse cell types within the embryo. In mammals, cells positioned inside the embryo give rise to the inner cell mass (ICM), which eventually forms the embryo itself. Yet, the molecular basis of how these cells recognise their 'inside' position to instruct their fate is unknown. Here, we show that provision of extracellular matrix (ECM) to isolated embryonic cells induces ICM specification and alters the subsequent spatial arrangement between epiblast (EPI) and primitive endoderm (PrE) cells that emerge within the ICM. Notably, this effect is dependent on integrin β1 activity and involves apical-to-basal conversion of cell polarity. We demonstrate that ECM-integrin activity is sufficient for 'inside' positional signalling and is required for correct EPI/PrE patterning. Thus, our findings highlight the significance of ECM-integrin adhesion in enabling position sensing by cells to achieve tissue patterning. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2022. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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