Action of carvacrol in Salmonella Typhimurium biofilm: A proteomic study.

Autor: Alves Coelho Trevisan D; State University of Maringa, Department of Clinical Analysis and Biomedicine, Maringa, Brazil., Aline Zanetti Campanerut-Sa P; State University of Maringa, Department of Clinical Analysis and Biomedicine, Maringa, Brazil., da Silva AF; State University of Minas Gerais, Department of Biological Sciences, Ituiutaba, Brasil., Farias Pereira Batista A; State University of Maringa, Department of Clinical Analysis and Biomedicine, Maringa, Brazil., Seixas FAV; State University of Maringa, Department of Technology, Umuarama, Brazil., Peralta RM; State University of Maringa , Department of Biochemistry, Maringa, Brazil., de Sa-Nakanishi AB; State University of Maringa , Department of Biochemistry, Maringa, Brazil., de Abreu Filho BA; State University of Maringa, Department of Basic Health Sciences, Maringa, Brazil., Machinski Junior M; State University of Maringa, Department of Basic Health Sciences, Maringa, Brazil., Graton Mikcha JM; State University of Maringa, Department of Clinical Analysis and Biomedicine, Maringa, Brazil.
Jazyk: angličtina
Zdroj: Journal of applied biomedicine [J Appl Biomed] 2020 Dec; Vol. 18 (4), pp. 106-114. Date of Electronic Publication: 2020 Sep 22.
DOI: 10.32725/jab.2020.014
Abstrakt: Carvacrol presents action in Salmonella Typhimurium biofilms, however the antibiofilm mechanism of this compound has not been fully established yet. In the present study, the aim was to evaluate protein profile changes in S. Typhimurium biofilm treated with carvacrol. Proteomic analysis of treated versus untreated biofilm showed several changes in proteins involved with S. Typhimurium biofilm and antioxidant activity. The proteins DsbA (thiol: disulfide interchange protein DsbA), LuxS (S-ribosylhomocysteine lyase), DksA (RNA polymerase binding transcription factor DksA), and SODs (superoxide dismutases) A, B and C had their synthesis decreased after treatment with carvacrol. These proteins play a key role in S. Typhimurium biofilm formation, demonstrating the dynamic antibiofilm action of carvacrol. The differentially expressed proteins identified provide possible action targets for future studies in order to gain more insight into the mechanism of action of carvacrol on S. Typhimurium biofilm.
Competing Interests: The authors report no conflicts of interest in this work.
Databáze: MEDLINE
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