Immunoreactivity of the SARS-CoV-2 entry proteins ACE-2 and TMPRSS-2 in murine models of hormonal manipulation, ageing, and cardiac injury.

Autor: Bengs S; Department of Nuclear Medicine, University Hospital Zurich, Zurich, Switzerland.; Center for Molecular Cardiology, University of Zurich, Schlieren, Switzerland., Rossi A; Department of Nuclear Medicine, University Hospital Zurich, Zurich, Switzerland.; Center for Molecular Cardiology, University of Zurich, Schlieren, Switzerland., Haberecker M; Institute of Pathology and Molecular Pathology, University Hospital Zurich, Zurich, Switzerland., Mikail N; Department of Nuclear Medicine, University Hospital Zurich, Zurich, Switzerland.; Center for Molecular Cardiology, University of Zurich, Schlieren, Switzerland., Meisel A; Department of Nuclear Medicine, University Hospital Zurich, Zurich, Switzerland.; Center for Molecular Cardiology, University of Zurich, Schlieren, Switzerland., Haider A; Division of Nuclear Medicine and Molecular Imaging, Massachusetts General Hospital, and Department of Radiology, Harvard Medical School, Boston, MA, USA., Grämer M; Department of Nuclear Medicine, University Hospital Zurich, Zurich, Switzerland.; Center for Molecular Cardiology, University of Zurich, Schlieren, Switzerland., Portmann A; Department of Nuclear Medicine, University Hospital Zurich, Zurich, Switzerland.; Center for Molecular Cardiology, University of Zurich, Schlieren, Switzerland., Todorov A; Department of Nuclear Medicine, University Hospital Zurich, Zurich, Switzerland.; Center for Molecular Cardiology, University of Zurich, Schlieren, Switzerland., Schönenberger C; Intensive Care Unit, University Hospital Basel, Basel, Switzerland., Gebhard CE; Intensive Care Unit, University Hospital Basel, Basel, Switzerland., Kuster GM; Department of Cardiology, University Hospital Basel, Basel, Switzerland.; Department of Biomedicine, Myocardial Research, University of Basel, Basel, Switzerland., Regitz-Zagrosek V; Charité, Universitätsmedizin Berlin, Berlin, Germany.; DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, Berlin, Germany., Gebhard C; Department of Nuclear Medicine, University Hospital Zurich, Zurich, Switzerland. catherine.gebhard@usz.ch.; Center for Molecular Cardiology, University of Zurich, Schlieren, Switzerland. catherine.gebhard@usz.ch.; Division of Cardiology, Department of Internal Medicine II, Medical University of Vienna, Vienna, Austria. catherine.gebhard@usz.ch.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Dec 14; Vol. 11 (1), pp. 23993. Date of Electronic Publication: 2021 Dec 14.
DOI: 10.1038/s41598-021-03181-3
Abstrakt: Previous work indicates that SARS-CoV-2 virus entry proteins angiotensin-converting enzyme 2 (ACE-2) and the cell surface transmembrane protease serine 2 (TMPRSS-2) are regulated by sex hormones. However, clinical studies addressing this association have yielded conflicting results. We sought to analyze the impact of sex hormones, age, and cardiovascular disease on ACE-2 and TMPRSS-2 expression in different mouse models. ACE-2 and TMPRSS-2 expression was analyzed by immunostaining in a variety of tissues obtained from FVB/N mice undergoing either gonadectomy or sham-surgery and being subjected to ischemia-reperfusion injury or transverse aortic constriction surgery. In lung tissues sex did not have a significant impact on the expression of ACE-2 and TMPRSS-2. On the contrary, following myocardial injury, female sex was associated to a lower expression of ACE-2 at the level of the kidney tubules. In addition, after myocardial injury, a significant correlation between younger age and higher expression of both ACE-2 and TMPRSS-2 was observed for lung alveoli and bronchioli, kidney tubules, and liver sinusoids. Our experimental data indicate that gonadal hormones and biological sex do not alter ACE-2 and TMPRSS-2 expression in the respiratory tract in mice, independent of disease state. Thus, sex differences in ACE-2 and TMPRSS-2 protein expression observed in mice may not explain the higher disease burden of COVID-19 among men.
(© 2021. The Author(s).)
Databáze: MEDLINE
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