A highly potential acyclic Schiff base fluorescent turn on sensor for Zn 2+ ions and colorimetric chemosensor for Zn 2+ , Cu 2+ and Co 2+ ions and its applicability in live cell imaging.

Autor:	Jayaraj A; Main group Organometallics Materials, Supramolecular Chemistry and Catalysis lab, Department of Chemistry, National Institute of Technology, Calicut 673601, India., Gayathri MS; Main group Organometallics Materials, Supramolecular Chemistry and Catalysis lab, Department of Chemistry, National Institute of Technology, Calicut 673601, India., Sivaraman G; Department of Chemistry, Gandhigram Rural Institute (Deemed to be University), Gandhigram 624302, India., P CAS; Main group Organometallics Materials, Supramolecular Chemistry and Catalysis lab, Department of Chemistry, National Institute of Technology, Calicut 673601, India. Electronic address: swamy@nitc.ac.in.
Jazyk:	angličtina
Zdroj:	Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2022 Jan; Vol. 226, pp. 112371. Date of Electronic Publication: 2021 Dec 02.
DOI:	10.1016/j.jphotobiol.2021.112371
Abstrakt:	Herein, we report two acyclic Schiff base receptors CS-1 and CS-2 capable of being selective fluorescent turn on for Zn ²⁺ ions and colorimetric chemosensor for Zn ²⁺ , Cu ²⁺ , and Co ²⁺ ions by showing a colour change from colourless to yellow in 1:1 ratio of acetonitrile and HEPES buffer (1:1, v/v, pH 7.4) without the interference from other metal ions screened (Cd ²⁺ , Hg ²⁺ , Sn ²⁺ , Ni ²⁺ , Cr ³⁺ , Mn ²⁺ , Pb ²⁺ , Ba ²⁺ , Al ³⁺ , Ca ²⁺ , Mg ²⁺ , K ⁺ and Na ⁺ ). The fluorescence turn on enhancement towards Zn ²⁺ ions is ascribed to PET blocking, suppression of -C=N- isomerisation, and the ESIPT process. The selectivity, competitivity and reversibility of the synthesised probes (CS-1 and CS-2) made them promising chemosensors for the detection of Zn ²⁺ , Cu ²⁺ , and Co ²⁺ ions. The density functional theory (DFT) calculations have theoretically endorsed the colorimetric changes in the examined absorption spectra and binding mode of both CS-1/CS-2 with metals ions. In addition, ¹ H NMR titrations were also consistent with the recognition mechanism of Zn ²⁺ ions with the CS-1/CS-2. Further, the Jobs plot analysis infers a 1:1 stoichiometric ratio for both evaluating receptors CS-1 and CS-2 with Zn ²⁺ , Cu ²⁺ and Co ²⁺ ions and was supported by DFT, NMR (only for Zn ²⁺ ions), UV-Visible, and fluorescence spectroscopic studies. Moreover, the detection limits of CS-1 and CS-2 for Zn ²⁺ ions were determined to be 7.69 and 5.35 nM, respectively, which is less compared to the detection limit of Cu ²⁺ , Co ²⁺ ions as well as the limit approved by the United State Environmental Protection Agency (US EPA). The probes CS-1 and CS-2 found to show high fluorescence quantum yields at pH = 7 during the titration with Zn ²⁺ as compared with other pHs (5-6 and 8-11). Gratifyingly, fluorescence microscopy imaging in HeLa cells revealed that the pair of receptors can be employed as an excellent fluorescent probe for the detection of Zn ²⁺ ions in living cells, indicating that this facile chemosensor has a huge potential in cellular imaging. (Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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