Molecular Histology Analysis of Cryopreserved Tissue Using Peptide/Protein MALDI-TOF Imaging Mass Spectrometry (MALDI-IMS).

Autor:	Iloro I; CIC bioGUNE, Proteomics Platform, Basque Research & Technology Alliance (BRTA), ProteoRed-ISCIII, CIBERehd, Bizkaia Science and Technology Park, Derio, Spain., Escobés I; CIC bioGUNE, Proteomics Platform, Basque Research & Technology Alliance (BRTA), ProteoRed-ISCIII, CIBERehd, Bizkaia Science and Technology Park, Derio, Spain., Azkargorta M; CIC bioGUNE, Proteomics Platform, Basque Research & Technology Alliance (BRTA), ProteoRed-ISCIII, CIBERehd, Bizkaia Science and Technology Park, Derio, Spain., Elortza F; CIC bioGUNE, Proteomics Platform, Basque Research & Technology Alliance (BRTA), ProteoRed-ISCIII, CIBERehd, Bizkaia Science and Technology Park, Derio, Spain. felortza@cicbiogune.es.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2420, pp. 177-190.
DOI:	10.1007/978-1-0716-1936-0_14
Abstrakt:	Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) has emerged as a powerful tool for analyzing the spatial distribution of peptides, small proteins, and other molecules within biological tissues. The obtained signals can be correlated with underlying tissue architecture, without any geometrical distortion, enabling the so-called molecular histology. Here, we analyzed cryopreserved tissue samples employing the MALDI-IMS for proteins and peptides. We used a nonstandard OCT-free cryo-slicing protocol, followed by Carnoy delipidation. Automated matrix spray was utilized to circumvent some of MALDI-IMS technology drawbacks in protein and peptide analysis. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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