Increased Levels of Plasma Extracellular Heat-Shock Proteins 60 and 70 kDa Characterized Early-Onset Neonatal Sepsis.

Autor: Canul-Euan AA; Department of Inmunobioquímica, Instituto Nacional de Perinatología (INPer), Ciudad de México, Mexico., Zúñiga-González G; Department of Neonatología, Instituto Nacional de Perinatología (INPer), Mexico City, Mexico., Palacios-Luna JE; Department of Inmunobioquímica, Instituto Nacional de Perinatología (INPer), Ciudad de México, Mexico., Maida-Claros R; Department of Neonatología, Instituto Nacional de Perinatología (INPer), Mexico City, Mexico., Díaz NF; Department of Fisiología y Desarrollo Celular, Instituto Nacional de Perinatología (INPer), Mexico City, Mexico., Saltigeral-Tigeral P; Instituto Nacional de Pediatría, Mexico City, Mexico., Karina García-May P; Servicio Recién Nacidos, Hospital Regional Lic. Adolfo López Mateos, Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado (ISSSTE), Mexico City, Mexico., Díaz-Ruiz O; Department of Pharmacology, Emory University School of Medicine, Atlanta, GA, United States., Flores-Herrera H; Department of Inmunobioquímica, Instituto Nacional de Perinatología (INPer), Ciudad de México, Mexico.
Jazyk: angličtina
Zdroj: Frontiers in pediatrics [Front Pediatr] 2021 Nov 25; Vol. 9, pp. 740274. Date of Electronic Publication: 2021 Nov 25 (Print Publication: 2021).
DOI: 10.3389/fped.2021.740274
Abstrakt: Background: Extracellular heat-shock proteins ( e Hsp) are highly conserved molecules that play an important role in inflammatory diseases and have been quantified in plasma from patients with infectious diseases, including sepsis. There is a constant search for dependable biochemical markers that, in combination with conventional methods, could deliver a prompt and reliable diagnosis of early-onset neonatal sepsis. Objective: We sought to assess the level of e Hsp-27, e Hsp-60, e Hsp-70, and tumor necrosis factor-alpha (TNFα) in plasma of healthy neonates at term and infants with early-onset neonatal sepsis. Methods: This study included 34 newborns that were classified as healthy neonates at term (blood samples from the umbilical cord, n = 23) or infants with early-onset neonatal sepsis (blood samples obtained from umbilical artery by standard sterile procedures before starting a systemic antibiotic intervention, n = 11). All blood samples were centrifuged, and the plasma recovered to determine e Hsp-27, e Hsp-60, e Hsp-70, and TNFα levels by ELISA. Results: Our results indicate that the level of e Hsp-27 in healthy neonates at term was 0.045 ± 0.024 pg/ml. This value decreased 2.5-fold in infants with early-onset neonate sepsis (0.019 ± 0.006 pg/ml, p = 0.004). In contrast, the levels of e Hsp-60 and e Hsp-70 in healthy neonates at term were 13.69 ± 5.3 and 4.03 ± 2.6 pg/ml, respectively. These protein levels increased significantly 1.8- and 1.9-fold in the plasma of infants with early-onset neonatal sepsis ( p ≤ 0.001). The level of TNFα in healthy neonates at term was 2.94 ± 0.46 pg/ml, with a 3.0-fold increase in infants with early-onset neonatal sepsis (8.96 ± 0.72 pm/ml, p ≤ 0.001). The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of e Hsp compared with that of C-reactive protein were 73.3, 60.0, 47.8, and 33.3%, respectively. Conclusion: This study demonstrated a consistent increase of e Hsp-60 and e Hsp-70 in the plasma of infants diagnosed with early-onset neonatal sepsis. These proteins showed higher sensitivity and specificity than C-reactive protein and blood culture test.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2021 Canul-Euan, Zúñiga-González, Palacios-Luna, Maida-Claros, Díaz, Saltigeral-Tigeral, Karina García-May, Díaz-Ruiz and Flores-Herrera.)
Databáze: MEDLINE