Ribo-Seq and RNA-Seq of TMA46 ( DFRP1 ) and GIR2 ( DFRP2 ) knockout yeast strains.

Autor:	Egorov AA; Phystech School of Biological and Medical Physics, Moscow Institute of Physics and Technology (State University), Dolgoprudny, 141700, Russian Federation.; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119234, Russian Federation.; Sirius University of Science and Technology, Sochi, 354340, Russian Federation., Makeeva DS; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119234, Russian Federation.; Sirius University of Science and Technology, Sochi, 354340, Russian Federation., Makarova NE; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119234, Russian Federation.; Sirius University of Science and Technology, Sochi, 354340, Russian Federation.; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, 119234, Russian Federation., Bykov DA; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119234, Russian Federation.; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russian Federation., Hrytseniuk YS; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119234, Russian Federation.; Department of Chemistry, Lomonosov Moscow State University, Moscow, 119234, Russian Federation., Mitkevich OV; Bach Institute of Biochemistry, FRC of Biotechnology of the Russian Academy of Sciences, Moscow, 119071, Russian Federation., Urakov VN; Bach Institute of Biochemistry, FRC of Biotechnology of the Russian Academy of Sciences, Moscow, 119071, Russian Federation., Alexandrov AI; Bach Institute of Biochemistry, FRC of Biotechnology of the Russian Academy of Sciences, Moscow, 119071, Russian Federation., Kulakovskiy IV; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119234, Russian Federation.; Sirius University of Science and Technology, Sochi, 354340, Russian Federation.; Institute of Protein Research, Russian Academy of Sciences, Pushchino, 142290, Russian Federation., Dmitriev SE; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119234, Russian Federation.; Sirius University of Science and Technology, Sochi, 354340, Russian Federation.; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, 119234, Russian Federation.; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russian Federation.
Jazyk:	angličtina
Zdroj:	F1000Research [F1000Res] 2021 Nov 16; Vol. 10, pp. 1162. Date of Electronic Publication: 2021 Nov 16 (Print Publication: 2021).
DOI:	10.12688/f1000research.74727.1
Abstrakt:	In eukaryotes, stalled and collided ribosomes are recognized by several conserved multicomponent systems, which either block protein synthesis in situ and resolve the collision locally, or trigger a general stress response. Yeast ribosome-binding GTPases RBG1 (DRG1 in mammals) and RBG2 (DRG2) form two distinct heterodimers with TMA46 (DFRP1) and GIR2 (DFRP2), respectively, both involved in mRNA translation. Accumulated evidence suggests that the dimers play partially redundant roles in elongation processivity and resolution of ribosome stalling and collision events, as well as in the regulation of GCN1-mediated signaling involved in ribosome-associated quality control (RQC). They also genetically interact with SLH1 (ASCC3) helicase, a key component of RQC trigger (RQT) complex disassembling collided ribosomes. Here, we present RNA-Seq and ribosome profiling (Ribo-Seq) data from S. cerevisiae strains with individual deletions of the TMA46 and GIR2 genes. Raw RNA-Seq and Ribo-Seq data as well as gene-level read counts are available in NCBI Gene Expression Omnibus (GEO) repository under GEO accession GSE185458 and GSE185286. Competing Interests: No competing interests were disclosed. (Copyright: © 2021 Egorov AA et al.)
Databáze:	MEDLINE
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