Agrobacterium tumefaciens Type IV and Type VI Secretion Systems Reside in Detergent-Resistant Membranes.

Autor:	Czolkoss S; Department of Microbial Biology, Ruhr University Bochum, Bochum, Germany., Safronov X; Department of Microbial Biology, Ruhr University Bochum, Bochum, Germany., Rexroth S; Department of Plant Biochemistry, Ruhr University Bochum, Bochum, Germany., Knoke LR; Department of Microbial Biology, Ruhr University Bochum, Bochum, Germany., Aktas M; Department of Microbial Biology, Ruhr University Bochum, Bochum, Germany., Narberhaus F; Department of Microbial Biology, Ruhr University Bochum, Bochum, Germany.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2021 Nov 25; Vol. 12, pp. 754486. Date of Electronic Publication: 2021 Nov 25 (Print Publication: 2021).
DOI:	10.3389/fmicb.2021.754486
Abstrakt:	Cell membranes are not homogenous but compartmentalized into lateral microdomains, which are considered as biochemical reaction centers for various physiological processes in eukaryotes and prokaryotes. Due to their special lipid and protein composition, some of these microdomains are resistant to treatment with non-ionic detergents and can be purified as detergent-resistant membranes (DRMs). Here we report the proteome of DRMs from the Gram-negative phytopathogen Agrobacterium tumefaciens . Using label-free liquid chromatography-tandem mass spectrometry, we identified proteins enriched in DRMs isolated under normal and virulence-mimicking growth conditions. Prominent microdomain marker proteins such as the SPFH (stomatin/prohibitin/flotillin/HflKC) proteins HflK, HflC and Atu3772, along with the protease FtsH were highly enriched in DRMs isolated under any given condition. Moreover, proteins involved in cell envelope biogenesis, transport and secretion, as well as motility- and chemotaxis-associated proteins were overrepresented in DRMs. Most strikingly, we found virulence-associated proteins such as the VirA/VirG two-component system, and the membrane-spanning type IV and type VI secretion systems enriched in DRMs. Fluorescence microscopy of the cellular localization of both secretion systems and of marker proteins was in agreement with the results from the proteomics approach. These findings suggest that virulence traits are micro-compartmentalized into functional microdomains in A. tumefaciens . Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Czolkoss, Safronov, Rexroth, Knoke, Aktas and Narberhaus.)
Databáze:	MEDLINE
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