[Establishment of a model of reproductive system injury in male Wistar rats in high-altitude hypoxia environment].

Autor:	Chang DH; Department of Urology, the 940th Hospital of PLA Joint Logistics Support Force, Lanzhou, Gansu 730050, China., Kong FY; Department of Urology, the 940th Hospital of PLA Joint Logistics Support Force, Lanzhou, Gansu 730050, China.; Department of Urology, Fengtai Hospital of Integrated Traditional Chinese and Western Medicine, Beijing 100072, China., Jiang W; Department of Recuperation 1, Hangzhou Special Service Sanatorial Center of PLA Air Force, Hangzhou, Zhejiang 310007, China., Li FD; Department of Urology, the 940th Hospital of PLA Joint Logistics Support Force, Lanzhou, Gansu 730050, China., Kang YD; Department of Urology, the 940th Hospital of PLA Joint Logistics Support Force, Lanzhou, Gansu 730050, China., Zhou X; Department of Urology, the 940th Hospital of PLA Joint Logistics Support Force, Lanzhou, Gansu 730050, China.; Gansu University of Chinese Medicine, Lanzhou, Gansu 730000, China., Ding HS; Department of Urology, the 940th Hospital of PLA Joint Logistics Support Force, Lanzhou, Gansu 730050, China.; Gansu University of Chinese Medicine, Lanzhou, Gansu 730000, China., Zhang B; Department of Urology, the 940th Hospital of PLA Joint Logistics Support Force, Lanzhou, Gansu 730050, China.
Jazyk:	čínština
Zdroj:	Zhonghua nan ke xue = National journal of andrology [Zhonghua Nan Ke Xue] 2020 Dec; Vol. 26 (12), pp. 1068-1073.
Abstrakt:	Objective: To establish a model of reproductive system injury in male rats at high altitude using the low-pressure hypoxic animal laboratory and study the changes in the testicular tissue, semen parameters, blood gas and oxidative stress in male rats at different altitudes. Methods: Sixty male Wistar rats were randomly assigned to be raised on the plains (the plains group, n = 20), at an altitude of 4 000 m (the plateau model group Ⅰ, n = 20), or at an altitude of 6 000 m (the plateau model group Ⅱ, n = 20) for a spermatogenic cycle of 14 days. After establishment of the model of high-altitude reproductive system injury, the testis tissues of the rats were harvested for HE staining and observed for histopathological changes under the light microscope, and their epididymedes collected for preparation of sperm suspension and detection of sperm motility, sperm count and the percentage of morphologically abnormal sperm (MAS). The blood gas level and oxidative stress-related indexes in different groups were also measured using the serological test. Results: With the elevation of altitude, the levels of pH and PO2 were decreased, those of PCO2, Hct, K+, Cl－ and Hb increased markedly, while that of Na+ exhibited no significant change. The model rats also showed folded spermatogenic tubule walls, thinned spermatogenic epithelia, disorderly arranged and reduced number of spermatogenic cells, and increased vascuolization in the spermatogenic epithelia, with decreased sperm motility and count, increased percentage of MAS, elevated concentration of malondialdehyde (MDA) and reduced activity of superoxide dismutase (SOD). Conclusions: A model of reproductive system injury was successfully established in male rats at a simulated altitude of 4 000 m. With increasing of the altitude to 6000 m, oxidative damage to the testicular tissue was aggravated, sperm motility decreased, and the percentage of MAS increased, indicating that an altitude of 6 000 m may cause serious damage to the rat reproductive system.
Databáze:	MEDLINE
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