Early structural and functional changes in Baikal Sculpin gills exposed to suspended soot microparticles in experiment.

Autor: Sudakov NP; Limnological Institute, Siberian Branch, Russian Academy of Sciences, 3 Ulan-Batorskaya St., Irkutsk, 664033, Russia. Electronic address: npsudakov@gmail.com., Klimenkov IV; Limnological Institute, Siberian Branch, Russian Academy of Sciences, 3 Ulan-Batorskaya St., Irkutsk, 664033, Russia., Bedoshvili YD; Limnological Institute, Siberian Branch, Russian Academy of Sciences, 3 Ulan-Batorskaya St., Irkutsk, 664033, Russia., Arsent'ev KY; Limnological Institute, Siberian Branch, Russian Academy of Sciences, 3 Ulan-Batorskaya St., Irkutsk, 664033, Russia., Gorshkov AG; Limnological Institute, Siberian Branch, Russian Academy of Sciences, 3 Ulan-Batorskaya St., Irkutsk, 664033, Russia., Izosimova ON; Limnological Institute, Siberian Branch, Russian Academy of Sciences, 3 Ulan-Batorskaya St., Irkutsk, 664033, Russia., Yakhnenko VM; Limnological Institute, Siberian Branch, Russian Academy of Sciences, 3 Ulan-Batorskaya St., Irkutsk, 664033, Russia., Kupchinskii AB; Baikal Museum, Siberian Branch, Russian Academy of Sciences, 1 Akademicheskaya St., Listvyanka, 664520, Russia., Didorenko SI; Baikal Museum, Siberian Branch, Russian Academy of Sciences, 1 Akademicheskaya St., Listvyanka, 664520, Russia., Likhoshway YV; Limnological Institute, Siberian Branch, Russian Academy of Sciences, 3 Ulan-Batorskaya St., Irkutsk, 664033, Russia.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2022 Mar; Vol. 290, pp. 133241. Date of Electronic Publication: 2021 Dec 09.
DOI: 10.1016/j.chemosphere.2021.133241
Abstrakt: The toxic influence of soot microparticles on terrestrial organisms has been well studied, although there is scarce data on how microparticles could affect hydrobionts. We performed a first-ever study of the short-term (5 days) impact of furnace soot (0.005 g/L) on the structural and functional features of gill cells in the Baikal Sculpin species Paracottus knerii, Dybowski, 1874. The soot samples used in the experiment were composed of small (10-100 nm) particles and larger (up to 20 μm) aggregates. The dominant fractions of the polycyclic aromatic hydrocarbons of these microparticles were phenanthrene, fluoranthene, pyrene, benzo[a]anthracene, chrysene, benzofluoranthenes, benzopyrenes, indeno[1,2,3-c,d]pyrenes, and benzo[ghi]perylene. Trace element analysis of the soot detected the presence of C, S, Si, Al, Ca, K, Mg, P, and Fe. The gill condition was assessed with electron scanning, transmission, and laser confocal microscopy. Soot induces degenerative changes in the macrostructure and surface of secondary lamellae and increases mucus production in fish gills. A decrease in mitochondrial activity, an increase in reactive oxygen species production, and an increase in the frequency of programmed cell death in gill epithelium were observed under the influence of soot. In chloride cells, an induction of macroautophagy was detected. In general, the changes in fish gills after the short-term influence of soot microparticles indicate the stress of respiratory and osmotic regulation systems in fish. The data obtained are important for forming a coherent picture of the impact of soot on hydrobionts and for developing bioindication methods for evaluating the risks of their influence on aquatic ecosystems.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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