Toxic effects of SiO 2 NPs in early embryogenesis of Xenopuslaevis.

Autor: Carotenuto R; Department of Biology, University of Naples Federico II, Naples, Italy. Electronic address: rosa.carotenuto@unina.it., Tussellino M; Department of Biology, University of Naples Federico II, Naples, Italy., Ronca R; Institute of Biostructures and Bioimaging (IBB)-CNR, Naples, Italy., Benvenuto G; Stazione Zoologica 'Anton Dohrn' Naples, Italy., Fogliano C; Department of Biology, University of Naples Federico II, Naples, Italy., Fusco S; Department of Medicine and Health Sciences 'Vincenzo Tiberio', University of Molise, Campobasso, Italy., Netti PA; Center for Advanced Biomaterials for Health Care (CABHC), Italian Institute of Technology, Naples, Italy; Interdisciplinary Research Centre on Biomaterials (CRIB), University of Naples Federico II, Naples, Italy; Department of Chemical Materials and Industrial Production (DICMAPI), University of Naples Federico II, Naples, Italy.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2022 Feb; Vol. 289, pp. 133233. Date of Electronic Publication: 2021 Dec 08.
DOI: 10.1016/j.chemosphere.2021.133233
Abstrakt: The exposure of organisms to the nanoparticulate is potentially hazardous, particularly when it occurs during embryogenesis. The effects of commercial SiO 2 NPs in early development were studied, using Xenopus laevis as a model to investigate their possible future employment by means of the Frog Embryo Teratogenesis Assay-Xenopus test (FETAX). The SiO 2 NPs did not change the survival but produced several abnormalities in developing embryos, in particular, the dorsal pigmentation, the cartilages of the head and branchial arches were modified; the encephalon, spinal cord and nerves are anomalous and the intestinal brush border show signs of suffering; these embryos are also bradycardic. In addition, the expression of genes involved in the early pathways of embryo development was modified. Treated embryos showed an increase of reactive oxygen species. This study suggests that SiO 2 NPs are toxic but non-lethal and showed potential teratogenic effects in Xenopus. The latter may be due to their cellular accumulation and/or to the effect caused by the interaction of SiO 2 NPs with cytoplasmic and/or nuclear components. ROS production could contribute to the observed effects. In conclusion, the data indicates that the use of SiO 2 NPs requires close attention and further studies to better clarify their activity in animals, including humans.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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