| Autor: |
Ramadan S; Department of Pharmacognosy, College of Pharmacy, Cairo University, Cairo, Egypt., Sabry MM; Department of Pharmacognosy, College of Pharmacy, Cairo University, Cairo, Egypt., Saad MA; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.; School of Pharmacy, New Giza University, Giza, Egypt., Angeloni S; School of Pharmacy, University of Camerino, Camerino, Italy.; RICH - Research and Innovation Coffee Hub, Macerata, Italy., Sabry OM; Department of Pharmacognosy, College of Pharmacy, Cairo University, Cairo, Egypt., Caprioli G; School of Pharmacy, University of Camerino, Camerino, Italy., El Zalabani SM; Department of Pharmacognosy, College of Pharmacy, Cairo University, Cairo, Egypt. |
| Abstrakt: |
Withania somnifera , Angelica sinensis, Glycyrrhiza glabra , and Simmondsia chinensis were acquired from the Egyptian market, profiled for their chemical constituents, screened for the in-vitro MAO-B inhibitory activity and evaluated for the total phenolic content. Thirty compounds were characterized in the selected herbs using HPLC-MS/MS. In-vitro MAO-B inhibitory activity and total phenolic content of the acquired herbs were compared with those of a prepared herbal formula consisting of a mixture of equal amounts of the four mentioned herbs. The most potent MAO-B inhibitory activity was exerted by the methanol extract of the prepared formula (IC 50 of 712.19 ± 13.90 ng/mL) compared to selegiline (IC 50 of 581.69 ± 11.35 ng/mL). The highest value of the total phenolic content was shown by Angelica sinensis methanolic extract (76.15 ± 0.1 mg/g) followed by Glycyrrhiza glabra methanolic extract (65.74 ± 0.1 mg/g), then the mixture's methanolic extract of the four herbs (37.04 ± 0.1 mg/g). |
