Using metabolomic profiling to inform use of surrogate species in ecological risk assessment practices.

Autor: Seim RF; Grantee to the U.S. EPA via Oak Ridge Institute for Science and Education, Athens, GA 30605, USA., Glinski DA; NRC Postdoctoral Research Fellow to the U.S. EPA, Athens, GA 30605, USA., Lavelle CM; U.S. EPA, ORD, CEMM, GEMMD, Gulf Breeze, FL 32561, USA., Awkerman JA; U.S. EPA, ORD, CEMM, GEMMD, Gulf Breeze, FL 32561, USA., Hemmer BL; U.S. EPA, ORD, CEMM, GEMMD, Gulf Breeze, FL 32561, USA., Harris P; U.S. EPA, ORD, CEMM, GEMMD, Gulf Breeze, FL 32561, USA., Raimondo S; U.S. EPA, ORD, CEMM, GEMMD, Gulf Breeze, FL 32561, USA., Snyder MN; Grantee to the U.S. EPA via Oak Ridge Institute for Science and Education, Athens, GA 30605, USA., Acrey BW; U.S. EPA, ORD, CEMM, EPD, Athens, GA 30605, USA., Purucker ST; U.S. EPA, ORD, CCTE, GLTED, RTP, NC 27709, USA., MacMillan DK; U.S. EPA, ORD, CCTE, CCED, RTP, NC 27009, USA., Brennan AA; U.S. EPA, ORD, CCTE, CCED, RTP, NC 27009, USA., Henderson WM; U.S. EPA, ORD, CEMM, EPD, Athens, GA 30605, USA. Electronic address: Henderson.Matt@epa.gov.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Part D, Genomics & proteomics [Comp Biochem Physiol Part D Genomics Proteomics] 2022 Mar; Vol. 41, pp. 100947. Date of Electronic Publication: 2021 Nov 29.
DOI: 10.1016/j.cbd.2021.100947
Abstrakt: The U.S. EPA frequently uses avian or fish toxicity data to set protective standards for amphibians in ecological risk assessments. However, this approach does not always adequately represent aquatic-dwelling and terrestrial-phase amphibian exposure data. For instance, it is accepted that early life stage tests for fish are typically sensitive enough to protect larval amphibians, however, metamorphosis from tadpole to a terrestrial-phase adult relies on endocrine cues that are less prevalent in fish but essential for amphibian life stage transitions. These differences suggest that more robust approaches are needed to adequately elucidate the impacts of pesticide exposure in amphibians across critical life stages. Therefore, in the current study, methodology is presented that can be applied to link the perturbations in the metabolomic response of larval zebrafish (Danio rerio), a surrogate species frequently used in ecotoxicological studies, to those of African clawed frog (Xenopus laevis) tadpoles following exposure to three high-use pesticides, bifenthrin, chlorothalonil, or trifluralin. Generally, D. rerio exhibited greater metabolic perturbations in both number and magnitude across the pesticide exposures as opposed to X. laevis. This suggests that screening ecological risk assessment surrogate toxicity data would sufficiently protect amphibians at the single life stage studied but care needs to be taken to understand the suite of metabolic requirements of each developing species. Ultimately, methodology presented, and data gathered herein will help inform the applicability of metabolomic profiling in establishing the risk pesticide exposure poses to amphibians and potentially other non-target species.
(Copyright © 2021. Published by Elsevier Inc.)
Databáze: MEDLINE
Externí odkaz: