Flow cytometric analysis of myelodysplasia: Pre-analytical and technical issues-Recommendations from the European LeukemiaNet.

Autor: van der Velden VHJ; Laboratory Medical Immunology, Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands., Preijers F; Department of Laboratory Medicine - Laboratory for Hematology, Radboudumc, Nijmegen, The Netherlands., Johansson U; Laboratory Medicine, SI-HMDS, University Hospitals Bristol and Weston NHS Foundation Trust, Bristol, UK., Westers TM; Department of Hematology, Amsterdam UMC, location VU University Medical Center, Cancer Center Amsterdam, Amsterdam, The Netherlands., Dunlop A; Department of Haemato-Oncology, Royal Marsden Hospital, Sutton, Surrey, UK., Porwit A; Department of Clinical Sciences, Division of Oncology And Pathology, Faculty of Medicine, Lund University, Lund, Sweden., Béné MC; Hematology Biology, Nantes University Hospital and CRCINA, Nantes, France., Valent P; Department of Internal Medicine I, Division of Hematology & Hemostaseology, Medical University of Vienna, Vienna, Austria.; Ludwig Boltzmann Institute for Hematology and Oncology, Medical University of Vienna, Vienna, Austria., Te Marvelde J; Laboratory Medical Immunology, Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands., Wagner-Ballon O; Department of Hematology and Immunology; and Université Paris-Est Créteil, Assistance Publique-Hôpitaux de Paris, University Hospital Henri Mondor, Inserm U955, Créteil, France., Oelschlaegel U; Department of Internal Medicine, University Hospital Carl-Gustav-Carus, Dresden, TU, Germany., Saft L; Department of Clinical Pathology and Oncology, Karolinska University Hospital and Institute, Solna, Stockholm, Sweden., Kordasti S; Comprehensive Cancer Centre, King's College London and Hematology Department, Guy's Hospital, London, UK., Ireland R; Comprehensive Cancer Centre, King's College London and Hematology Department, Guy's Hospital, London, UK., Cremers E; Department of Internal Medicine, Division of Hematology, Maastricht University Medical Center, AZ, Maastricht, The Netherlands., Alhan C; Department of Hematology, Amsterdam UMC, location VU University Medical Center, Cancer Center Amsterdam, Amsterdam, The Netherlands., Duetz C; Department of Hematology, Amsterdam UMC, location VU University Medical Center, Cancer Center Amsterdam, Amsterdam, The Netherlands., Hobo W; Department of Laboratory Medicine - Laboratory for Hematology, Radboudumc, Nijmegen, The Netherlands., Chapuis N; Assistance Publique-Hôpitaux de Paris. Centre-Université de Paris, Cochin Hospital, Laboratory of Hematology and Université de Paris, Institut Cochin, INSERM U1016, CNRS UMR8104, Paris, France., Fontenay M; Assistance Publique-Hôpitaux de Paris. Centre-Université de Paris, Cochin Hospital, Laboratory of Hematology and Université de Paris, Institut Cochin, INSERM U1016, CNRS UMR8104, Paris, France., Bettelheim P; Department of Internal Medicine, Ordensklinikum Linz Barmherzige Schwestern - Elisabethinen, Linz, Austria., Eidenshink-Brodersen L; Hematologics Inc, Seattle, WA, USA., Font P; Department of Hematology, Hospital General Universitario Gregorio Marañon-IiSGM, Madrid, Spain., Loken MR; Hematologics Inc, Seattle, WA, USA., Matarraz S; Cancer Research Center (IBMCC, USAL-CSIC), Department of Medicine and Cytometry Service, University of Salamanca, Institute for Biomedical Research of Salamanca (IBSAL), Salamanca, Spain.; Biomedical Research Networking Centre Consortium of Oncology (CIBERONC), Instituto Carlos III, Salamanca, Spain., Ogata K; Metropolitan Research and Treatment Centre for Blood Disorders (MRTC Japan), Tokyo, Japan., Orfao A; Cancer Research Center (IBMCC, USAL-CSIC), Department of Medicine and Cytometry Service, University of Salamanca, Institute for Biomedical Research of Salamanca (IBSAL), Salamanca, Spain.; Biomedical Research Networking Centre Consortium of Oncology (CIBERONC), Instituto Carlos III, Salamanca, Spain., Psarra K; Immunology Histocompatibility Department, Evangelismos Hospital, Athens, Greece., Subirá D; Flow Cytometry Unit. Department of Hematology, Hospital Universitario de Guadalajara, Guadalajara, Spain., Wells DA; Hematologics Inc, Seattle, WA, USA., Della Porta MG; IRCCS Humanitas Research Hospital, Rozzano, Milan, Italy & Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Milan, Italy., Burbury K; Department of Haematology, Peter MacCallum Cancer Centre, University of Melbourne, Melbourne, Australia., Bellos F; MLL Munich Leukemia Laboratory, Munich, Germany., Weiß E; MLL Munich Leukemia Laboratory, Munich, Germany., Kern W; MLL Munich Leukemia Laboratory, Munich, Germany., van de Loosdrecht A; Department of Hematology, Amsterdam UMC, location VU University Medical Center, Cancer Center Amsterdam, Amsterdam, The Netherlands.
Jazyk: angličtina
Zdroj: Cytometry. Part B, Clinical cytometry [Cytometry B Clin Cytom] 2023 Jan; Vol. 104 (1), pp. 15-26. Date of Electronic Publication: 2021 Dec 11.
DOI: 10.1002/cyto.b.22046
Abstrakt: Background: Flow cytometry (FCM) aids the diagnosis and prognostic stratification of patients with suspected or confirmed myelodysplastic syndrome (MDS). Over the past few years, significant progress has been made in the FCM field concerning technical issues (including software and hardware) and pre-analytical procedures.
Methods: Recommendations are made based on the data and expert discussions generated from 13 yearly meetings of the European LeukemiaNet international MDS Flow working group.
Results: We report here on the experiences and recommendations concerning (1) the optimal methods of sample processing and handling, (2) antibody panels and fluorochromes, and (3) current hardware technologies.
Conclusions: These recommendations will support and facilitate the appropriate application of FCM assays in the diagnostic workup of MDS patients. Further standardization and harmonization will be required to integrate FCM in MDS diagnostic evaluations in daily practice.
(© 2021 The Authors. Cytometry Part B: Clinical Cytometry published by Wiley Periodicals LLC on behalf of International Clinical Cytometry Society.)
Databáze: MEDLINE
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