Impact of Prematurity on the Buccal Epithelial Cells of the Neonates via Wnt/Beta-Catenin Signaling Pathway and Apoptosis.

Autor:	Donmez HG; Department of Biology, Faculty of Science, Hacettepe University, Ankara, Turkey., Celik HT; Division of Neonatology, Department of Pediatrics, Faculty of Science, Hacettepe University, Ankara, Turkey., Kayki G; Division of Neonatology, Department of Pediatrics, Faculty of Science, Hacettepe University, Ankara, Turkey., Tanacan A; Division of Perinatology, Department of Obstetrics and Gynecology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.; Department of Obstetrics and Gynecology, Turkish Ministry of Health Ankara City Hospital, Ankara, Turkey., Cagan M; Division of Perinatology, Department of Obstetrics and Gynecology, Faculty of Medicine, Hacettepe University, Ankara, Turkey., Yigit S; Division of Neonatology, Department of Pediatrics, Faculty of Science, Hacettepe University, Ankara, Turkey., Yurdakok M; Division of Neonatology, Department of Pediatrics, Faculty of Science, Hacettepe University, Ankara, Turkey., Cakar AN; Department of Histology, Faculty of Medicine, TOBB University, Ankara, Turkey., Beksac MS; Division of Perinatology, Department of Obstetrics and Gynecology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
Jazyk:	angličtina
Zdroj:	American journal of perinatology [Am J Perinatol] 2024 Mar; Vol. 41 (4), pp. 445-451. Date of Electronic Publication: 2021 Dec 10.
DOI:	10.1055/s-0041-1740348
Abstrakt:	Objective: Understanding the reflections of prematurity is necessary for the management of neonatal complications. We focused on the impact of prematurity and related "maternal risk factors/obstetric complications" on buccal cells of the neonates via evaluation of the Wnt/β-catenin signaling pathway and apoptosis. Study Design: This study consisted of "early preterm neonates (EPN) (≤34th gestational week [gw]) ( n  = 36)," "late preterm neonates (LPN) (34th- < 37th gw) ( n  = 46)," and "term neonates (control) (≥37th gw) ( n  = 56)." Cohort was also subclassified according to the presence of maternal risk factors, obstetric complications, and neonatal complications. Wnt/β-catenin signaling and caspase-3 activation pathways were studied immunocytochemically. Results: Wnt/β-catenin signaling positivity was statistically more frequent at buccal smears of the EPN and LPN groups compared with controls ( p  < 0.001). The cutoff for gestational age at delivery in receiver operating characteristic curve with the best balance of sensitivity (67.4%) and specificity (67.3%) was 35.8th gw for determining the reduction of Wnt/β-catenin signaling positivity ( p  < 0.001). The study demonstrated that obstetric complications significantly affected the activity of signaling, while maternal risk factors do not have any effect on Wnt/β-catenin signaling pathway ( p  = 0.003 and p  = 0.828, respectively). This study also demonstrated a significant relationship between Wnt/β-catenin signaling pathway and the presence of neonatal complications ( p  = 0.015). Conclusion: Dynamic characteristics of buccal cells are influenced by prematurity and related obstetric and neonatal problems. Buccal smear is a good tool to investigate the impact of prematurity and obstetric problems on perinatal outcome. Key Points: · Neonatal buccal cells are affected by prematurity and related obstetric/neonatal problems.. · 35.8th gw is critical for determining the reduction of Wnt/β-catenin signaling positivity.. · Obstetric and neonatal complications significantly related to Wnt/β-catenin signaling activity.. Competing Interests: None declared. (Thieme. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu