Untargeted metabolomics of the alkaliphilic cyanobacterium Plectonema terebrans elucidated novel stress-responsive metabolic modulations.

Autor: Shahid A; Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Faisalabad 38000, Pakistan., Siddiqui AJ; Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan., Musharraf SG; H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan., Liu CG; State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China. Electronic address: cg.liu@sjtu.edu.cn., Malik S; Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Faisalabad 38000, Pakistan., Syafiuddin A; Department of Public Health, Universitas Nahdlatul Ulama Surabaya, 60237 Surabaya, East Java, Indonesia., Boopathy R; Department of Biological Sciences, Nicholls State University, Thibodaux, LA 70310, USA., Tarbiah NI; Biochemistry Department, King Abdulaziz University, Jeddah, Saudi Arabia., Gull M; Biochemistry Department, King Abdulaziz University, Jeddah, Saudi Arabia., Mehmood MA; School of Bioengineering, Sichuan University of Science and Engineering, Zigong, China; Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Faisalabad 38000, Pakistan. Electronic address: draamer@gcuf.edu.pk.
Jazyk: angličtina
Zdroj: Journal of proteomics [J Proteomics] 2022 Feb 10; Vol. 252, pp. 104447. Date of Electronic Publication: 2021 Dec 07.
DOI: 10.1016/j.jprot.2021.104447
Abstrakt: Alkaliphilic cyanobacteria are suitable candidates to study the effect of alkaline wastewater cultivation on molecular metabolic responses. In the present study, the impact of wastewater, alkalinity, and alkaline wastewater cultivation was studied on the biomass production, biochemical composition, and the alkalinity responsive molecular mechanism through metabolomics. The results suggested a 1.29 to 1.44-fold higher biomass production along with improved lipid, carbohydrate, and pigment production under alkaline wastewater cultivation. The metabolomics analysis showed 1.2-fold and 5.54-fold increase in the indole-acetic acid and phytoene biosynthesis which contributed to overall enhanced cell differentiation and photo-protectiveness. Furthermore, lower levels of Ribulose-1,5-bisphosphate (RuBP), and higher levels of 2-phosphoglycerate and 3-phosphoglycerate suggested the efficient fixation of CO 2 into biomass, and storage compounds including polysaccharides, lipids, and sterols. Interestingly, except L-histidine and L-phenylalanine, all the metabolites related to protein biosynthesis were downregulated in response to wastewater and alkaline wastewater cultivation. The cells protected themselves from alkalinity and nutrient stress by improving the biosynthesis of sterols, non-toxic antioxidants, and osmo-protectants. Alkaline wastewater cultivation regulated the activation of carbon concentration mechanism (CCM), glycolysis, fatty-acid biosynthesis, and shikimate pathway. The data revealed the importance of alkaline wastewater cultivation for improved CO 2 fixation, wastewater treatment, and producing valuable bioproducts including phytoene, Lyso PC 18:0, and sterols. These metabolic pathways could be future targets of metabolic engineering for improving biomass and metabolite production. SIGNIFICANCE: Alkalinity is an imperative factor, responsible for the contamination control and biochemical regulation in cyanobactera, especially during the wastewater cultivation. Currently, understanding of alkaline wastewater responsive molecular mechanism is lacking and most of the studies are focused on transcriptomics of model organisms for this purpose. In this study, untargeted metabolomics was employed to analyze the impact of wastewater and alkaline wastewater on the growth, CO 2 assimilation, nutrient uptake, and associated metabolic modulations of the alkaliphilic cyanobacterium Plectonema terebrans BERC10. Results unveiled that alkaline wastewater cultivation regulated the activation of carbon concentration mechanism (CCM), glycolysis, fatty-acid biosynthesis, and shikimate pathway. It indicated the feasibility of alkaline wastewater as promising low-cost media for cyanobacterium cultivation. The identified stress-responsive pathways could be future genetic targets for strain improvement.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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