| Autor: |
DeBrosse M; Novel Device Lab., University of Cincinnati, Cincinnati, OH 45221, USA.; 711th Human Performance Wing, Air Force Research Laboratory, Wright-Patterson AFB, Dayton, OH 45433, USA., Yuan Y; Novel Device Lab., University of Cincinnati, Cincinnati, OH 45221, USA., Brothers M; 711th Human Performance Wing, Air Force Research Laboratory, Wright-Patterson AFB, Dayton, OH 45433, USA., Karajic A; Novel Device Lab., University of Cincinnati, Cincinnati, OH 45221, USA., van Duren J; Diamond Foundry Inc., South San Francisco, CA 94080, USA., Kim S; 711th Human Performance Wing, Air Force Research Laboratory, Wright-Patterson AFB, Dayton, OH 45433, USA., Hussain S; 711th Human Performance Wing, Air Force Research Laboratory, Wright-Patterson AFB, Dayton, OH 45433, USA., Heikenfeld J; Novel Device Lab., University of Cincinnati, Cincinnati, OH 45221, USA. |
| Abstrakt: |
Electrochemical biosensors promise a simple method to measure analytes for both point-of-care diagnostics and continuous, wearable biomarker monitors. In a liquid environment, detecting the analyte of interest must compete with other solutes that impact the background current, such as redox-active molecules, conductivity changes in the biofluid, water electrolysis, and electrode fouling. Multiple methods exist to overcome a few of these challenges, but not a comprehensive solution. Presented here is a combined boron-doped diamond electrode and oil-membrane protection approach that broadly mitigates the impact of biofluid interferents without a biorecognition element. The oil-membrane blocks the majority of interferents in biofluids that are hydrophilic while permitting passage of important hydrophobic analytes such as hormones and drugs. The boron-doped diamond then suppresses water electrolysis current and maintains peak electrochemical performance due to the foulant-mitigation benefits of the oil-membrane protection. Results show up to a 365-fold reduction in detection limits using the boron-doped diamond electrode material alone compared with traditional gold in the buffer. Combining the boron-doped diamond material with the oil-membrane protection scheme maintained these detection limits while exposed to human serum for 18 h. |
