Association of Ovar-DRB1 alleles with innate immune responses in sheep.

Autor: Esmailnejad A; Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran., Ganjiani V; Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran., Hosseini-Nasab E; Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran., Nazifi S; Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Jazyk: angličtina
Zdroj: Veterinary medicine and science [Vet Med Sci] 2022 Mar; Vol. 8 (2), pp. 752-757. Date of Electronic Publication: 2021 Dec 09.
DOI: 10.1002/vms3.683
Abstrakt: Background: Major histocompatibility complex (MHC) is the best characterised genetic region associated with adaptive immune responses, including humoral and cell-mediated immunities.
Objectives: In this study, the association of MHC class II alleles with inflammatory cytokines and acute-phase proteins was evaluated in sheep population.
Methods: Allelic diversity of second exon of ovine DRB1 locus (Ovar-DRB1.2) was determined in 100 indigenous Iranian Lori-Bakhtiari fat-tailed sheep using restriction fragment length polymorphism and direct sequencing methods. The association of DRB1.2 alleles with inflammatory cytokines (interleukin-1β, IL-1β; IL-6 and tumour necrosis factor-α) and acute-phase proteins (serum amyloid A, alpha-1-acid glycoprotein and haptoglobin) was examined using generalised linear model and multivariate regression analysis.
Results: Seven distinct RsaI restriction patterns and fourteen alleles were identified in this population. Allele DRB1*2101 showed a negative influence on the IL-6 response and was associated with lower serum level of IL-6. DRB1.2 heterozygous individuals also showed higher haptoglobin concentration than homozygotes.
Conclusions: These results provide additional support for the association between Ovar-DRB1 alleles and regulation of immune responses in sheep population. Description of MHC polymorphism and its role in the controlling of immune responses will increase our understanding of host-pathogen interactions, and ultimately facilitate the selection of disease-resistant flocks in genetic breeding programs.
(© 2021 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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