Natural self-emulsifying reversible hybrid-hydrogel delivery (N'SERH) of tocopherol enhances bioavailability and modulates alcohol-induced reproductive toxicity in rats.
| Autor: | Vismaya KU; Department of Zoology, Government Victoria College, Palakkad, Kerala, India., Noorjasmine TN; Department of Zoology, Government Victoria College, Palakkad, Kerala, India., Syam Das S; R&D Centre Akay Natural Ingredients, Cochin, Kerala, India., Kesavan L; Molecular Neurobiology Division, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India., Baby Chakrapani PS; Centre for Neuroscience, Cochin University of Science and Technology, Kochi, Kerala, India.; Department of Biotechnology, Cochin University of Science and Technology, Kochi, Kerala, India., Krishnakumar IM; R&D Centre Akay Natural Ingredients, Cochin, Kerala, India., Kumar CVS; Department of Zoology, Government Victoria College, Palakkad, Kerala, India. |
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| Jazyk: | angličtina |
| Zdroj: | Andrologia [Andrologia] 2022 Mar; Vol. 54 (2), pp. e14305. Date of Electronic Publication: 2021 Dec 08. |
| DOI: | 10.1111/and.14305 |
| Abstrakt: | Alpha-tocopherol (α-Toc), an antioxidant vitamin, has been widely prescribing in the treatment of infertility, in spite of its limited oral bioavailability. The present study describes the enhanced bioavailability and efficacy of a novel 'natural self-emulsifying reversible hydrogel' (N'SERH)-based oral delivery form of α-Toc-rich sunflower oil (Tα-fen) using fenugreek galactomannan hydrogel scaffold (hybrid-FENUMAT TM ). Tα-fen was characterised by FTIR, SEM, TEM and DLS as a hybrid-hydrogel powder. The bioavailability study on thirty (n = 30) male Sprague Dawley rats randomised into two groups indicated 4.84-fold increase in the oral bioavailability when the formulation was provided at 15 mg/kg b. wt. of α-Toc by oral gavage. The efficacy study on 24 animals randomised into four groups as control, ethanol treated (4 mg/kg b. wt.), ethanol+unformulated, UTα (15 mg/kg b. wt.) and ethanol+formulation, Tα-fen (15 mg/kg b. wt.) revealed significant improvement (*p < 0.05) and reversal of alcohol-induced reproductive toxicity as evident from the enhanced sperm count, motility and viability parameters, testosterone levels, fructose content, and SDH activity and plasma antioxidant status among Tα-fen-treated rats, compared with unformulated, UTα-treated group. Histopathology further confirmed the reversal of the alterations in the testes morphology of Tα-fen-treated animals, indicating its promising potential in the treatment of reproductive health issues. (© 2021 Wiley-VCH GmbH.) |
| Databáze: | MEDLINE |
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