Rapid and robust optogenetic control of gene expression in Drosophila.

Autor:	di Pietro F; Institut Curie, Université PSL, Sorbonne Université, CNRS UMR 3215, Inserm U934, Genetics and Developmental Biology, 75005 Paris, France., Herszterg S; Francis Crick Institute, 1 Midland Rd, London NW1 1AT, UK., Huang A; Francis Crick Institute, 1 Midland Rd, London NW1 1AT, UK., Bosveld F; Institut Curie, Université PSL, Sorbonne Université, CNRS UMR 3215, Inserm U934, Genetics and Developmental Biology, 75005 Paris, France., Alexandre C; Francis Crick Institute, 1 Midland Rd, London NW1 1AT, UK., Sancéré L; Institut Curie, Université PSL, Sorbonne Université, CNRS UMR 3215, Inserm U934, Genetics and Developmental Biology, 75005 Paris, France., Pelletier S; Institut Curie, Université PSL, Sorbonne Université, CNRS UMR 3215, Inserm U934, Genetics and Developmental Biology, 75005 Paris, France., Joudat A; Institut Curie, Université PSL, Sorbonne Université, CNRS UMR 3215, Inserm U934, Genetics and Developmental Biology, 75005 Paris, France., Kapoor V; Institut Curie, Université PSL, Sorbonne Université, CNRS UMR 3215, Inserm U934, Genetics and Developmental Biology, 75005 Paris, France., Vincent JP; Francis Crick Institute, 1 Midland Rd, London NW1 1AT, UK. Electronic address: jp.vincent@crick.ac.uk., Bellaïche Y; Institut Curie, Université PSL, Sorbonne Université, CNRS UMR 3215, Inserm U934, Genetics and Developmental Biology, 75005 Paris, France. Electronic address: yohanns.bellaiche@curie.fr.
Jazyk:	angličtina
Zdroj:	Developmental cell [Dev Cell] 2021 Dec 20; Vol. 56 (24), pp. 3393-3404.e7. Date of Electronic Publication: 2021 Dec 07.
DOI:	10.1016/j.devcel.2021.11.016
Abstrakt:	Deciphering gene function requires the ability to control gene expression in space and time. Binary systems such as the Gal4/UAS provide a powerful means to modulate gene expression and to induce loss or gain of function. This is best exemplified in Drosophila, where the Gal4/UAS system has been critical to discover conserved mechanisms in development, physiology, neurobiology, and metabolism, to cite a few. Here we describe a transgenic light-inducible Gal4/UAS system (ShineGal4/UAS) based on Magnet photoswitches. We show that it allows efficient, rapid, and robust activation of UAS-driven transgenes in different tissues and at various developmental stages in Drosophila. Furthermore, we illustrate how ShineGal4 enables the generation of gain and loss-of-function phenotypes at animal, organ, and cellular levels. Thanks to the large repertoire of UAS-driven transgenes, ShineGal4 enriches the Drosophila genetic toolkit by allowing in vivo control of gene expression with high temporal and spatial resolutions. Competing Interests: Declaration of interests Yohanns Bellaiche is a Developmental Cell advisory board member. (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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