Next-Generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire.

Autor: Melani RD; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States., Des Soye BJ; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.; Proteomics Center of Excellence, Evanston, Illinois 60208, United States., Kafader JO; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States., Forte E; Proteomics Center of Excellence, Evanston, Illinois 60208, United States.; Department of Surgery, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, United States., Hollas M; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States., Blagojevic V; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States., Negrão F; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States., McGee JP; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States., Drown B; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States., Lloyd-Jones C; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States., Seckler HS; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States., Camarillo JM; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States., Compton PD; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.; Integrated Protein Technologies, Evanston, Illinois 60201, United States., LeDuc RD; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States., Early B; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States., Fellers RT; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States., Cho BK; Proteomics Center of Excellence, Evanston, Illinois 60208, United States., Mattamana BB; Proteomics Center of Excellence, Evanston, Illinois 60208, United States., Goo YA; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.; Proteomics Center of Excellence, Evanston, Illinois 60208, United States., Thomas PM; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.; Proteomics Center of Excellence, Evanston, Illinois 60208, United States., Ash MK; Department of Microbial Pathogens and Immunity, Rush University Medical Center, Chicago, Illinois 60612, United States., Bhimalli PP; Department of Microbial Pathogens and Immunity, Rush University Medical Center, Chicago, Illinois 60612, United States., Al-Harthi L; Department of Microbial Pathogens and Immunity, Rush University Medical Center, Chicago, Illinois 60612, United States., Sha BE; Division of Infectious Diseases, Rush University Medical Center, Chicago, Illinois 60612, United States., Schneider JR; Department of Microbial Pathogens and Immunity, Rush University Medical Center, Chicago, Illinois 60612, United States., Kelleher NL; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.; Proteomics Center of Excellence, Evanston, Illinois 60208, United States.; Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611, United States.
Jazyk: angličtina
Zdroj: Journal of proteome research [J Proteome Res] 2022 Jan 07; Vol. 21 (1), pp. 274-288. Date of Electronic Publication: 2021 Dec 08.
DOI: 10.1021/acs.jproteome.1c00882
Abstrakt: Methods of antibody detection are used to assess exposure or immunity to a pathogen. Here, we present Ig-MS, a novel serological readout that captures the immunoglobulin (Ig) repertoire at molecular resolution, including entire variable regions in Ig light and heavy chains. Ig-MS uses recent advances in protein mass spectrometry (MS) for multiparametric readout of antibodies, with new metrics like Ion Titer (IT) and Degree of Clonality (DoC) capturing the heterogeneity and relative abundance of individual clones without sequencing of B cells. We applied Ig-MS to plasma from subjects with severe and mild COVID-19 and immunized subjects after two vaccine doses, using the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 as the bait for antibody capture. Importantly, we report a new data type for human serology, that could use other antigens of interest to gauge immune responses to vaccination, pathogens, or autoimmune disorders.
Databáze: MEDLINE