L-carnitine added to post-thawed semen acts as an antioxidant and a stimulator of equine sperm metabolism.

Autor: Lagares MA; Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., da Silva GC; Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., Cortes SF; Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., Moreira FHM; Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., Neves FCD; Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., Alves NC; Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., Viegas RN; Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., Diniz TF; Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., Lemos VS; Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., de Rezende ASC; Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., Freitas MM; Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil., Stahlberg R; Faculty of Veterinary Medicine, Pontifical Catholic University (PUC- Minas) of Minas Gerais, Betim, Brazil., Nicolino RR; Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil.
Jazyk: angličtina
Zdroj: Andrologia [Andrologia] 2022 Apr; Vol. 54 (3), pp. e14338. Date of Electronic Publication: 2021 Dec 08.
DOI: 10.1111/and.14338
Abstrakt: The objective of this study was to enhance the in vitro sperm quality and in vivo fertility of frozen-thawed equine semen by the addition of l-carnitine (LC) to post-thawed semen. Different concentrations of LC were added to thawed samples to obtain four treatments control and 0.5, 1 and 2 mM LC. In the in vitro experiments, sperm motility and kinematics, membrane integrity and intracellular calcium ion concentration ([Ca 2+ ] i ) were investigated, and the antioxidant bioactivity of LC was assessed by measuring hydrogen peroxide and nitrite concentrations (NO 2 - ). The fertility rate was assessed via the artificial insemination of mares. The treatment with 1 mM LC increased sperm [Ca 2+ ] i (60.6 ± 0.05 AU), reduced nitrite concentration (39.1 ± 14.9 µM/µg protein), increased the sperm straightness percentage (STR: 78.3 ± 5.3%) and increased the pregnancy rate (75%) as compared to the control ([Ca 2+ ] i 48.4 ± 0.05 AU, NO 2 - concentration 63.1 ± 14.4 µM/µg protein, STR 67.5 ± 7.9%, 12.5% pregnancy rate, p < 0.05). These results suggest that 1 mM LC acts as an antioxidant and stimulator of sperm metabolism in post-thawed equine semen, increasing the fertility rate. Thus, addition of LC might be an alternative to improve the fertility of poor quality post-thawed equine semen.
(© 2021 Wiley-VCH GmbH.)
Databáze: MEDLINE
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