| Autor: |
Balhaddad AA; Dental Biomedical Sciences Ph.D. Program, University of Maryland School of Dentistry, Baltimore, Maryland 21201, United States.; Department of Restorative Dental Sciences, Imam Abdulrahman Bin Faisal University, College of Dentistry, Dammam 31441, Saudi Arabia., Xia Y; Biomaterials & Tissue Engineering Division, Dept. of Advanced Oral Sciences and Therapeutics, University of Maryland School of Dentistry, Baltimore, Maryland 21201, United States.; Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, Jiangsu 210029, China.; Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China., Lan Y; Department of Physics and Engineering Physics, Morgan State University, 1700 East Cold Spring Lane, Baltimore, Maryland 21251, United States., Mokeem L; Dental Biomedical Sciences Ph.D. Program, University of Maryland School of Dentistry, Baltimore, Maryland 21201, United States., Ibrahim MS; Dental Biomedical Sciences Ph.D. Program, University of Maryland School of Dentistry, Baltimore, Maryland 21201, United States.; Department of Preventive Dental Sciences, Imam Abdulrahman Bin Faisal University, College of Dentistry, Dammam 31441, Saudi Arabia., Weir MD; Dental Biomedical Sciences Ph.D. Program, University of Maryland School of Dentistry, Baltimore, Maryland 21201, United States.; Biomaterials & Tissue Engineering Division, Dept. of Advanced Oral Sciences and Therapeutics, University of Maryland School of Dentistry, Baltimore, Maryland 21201, United States., Xu HHK; Dental Biomedical Sciences Ph.D. Program, University of Maryland School of Dentistry, Baltimore, Maryland 21201, United States.; Biomaterials & Tissue Engineering Division, Dept. of Advanced Oral Sciences and Therapeutics, University of Maryland School of Dentistry, Baltimore, Maryland 21201, United States., Melo MAS; Dental Biomedical Sciences Ph.D. Program, University of Maryland School of Dentistry, Baltimore, Maryland 21201, United States.; Division of Operative Dentistry, Dept. of General Dentistry, University of Maryland School of Dentistry, Baltimore, Maryland 21201, United States. |
| Abstrakt: |
Conventional antibiotic therapies for biofilm-trigged oral diseases are becoming less efficient due to the emergence of antibiotic-resistant bacterial strains. Antimicrobial photodynamic therapy (aPDT) is hampered by restricted access to bacterial communities embedded within the dense extracellular matrix of mature biofilms. Herein, a versatile photosensitizer nanoplatform (named MagTBO) was designed to overcome this obstacle by integrating toluidine-blue ortho (TBO) photosensitizer and superparamagnetic iron oxide nanoparticles (SPIONs) via a microemulsion method. In this study, we reported the preparation, characterization, and application of MagTBO for aPDT. In the presence of an external magnetic field, the MagTBO microemulsion can be driven and penetrate deep sites inside the biofilms, resulting in an improved photodynamic disinfection effect compared to using TBO alone. Besides, the obtained MagTBO microemulsions revealed excellent water solubility and stability over time, enhanced the aPDT performance against S. mutans and saliva-derived multispecies biofilms, and improved the TBO's biocompatibility. Such results demonstrate a proof-of-principle for using microemulsion as a delivery vehicle and magnetic field as a navigation approach to intensify the antibacterial action of currently available photosensitizers, leading to efficient modulation of pathogenic oral biofilms. |
