DHA-containing phospholipids control membrane fusion and transcellular tunnel dynamics.

Autor:	Tsai MC; Institut de Pharmacologie Moléculaire et Cellulaire, UMR 7275, CNRS and Université Côte d'Azur, 06560 Valbonne, France.; Institut Pasteur, Université de Paris, CNRS UMR6047, Inserm U1306, Unité des Toxines Bactériennes, 75015 Paris, France., Fleuriot L; Institut de Pharmacologie Moléculaire et Cellulaire, UMR 7275, CNRS and Université Côte d'Azur, 06560 Valbonne, France., Janel S; Université de Lille, CNRS, Inserm, CHU Lille, Institut Pasteur Lille, U1019 - UMR 9017 - CIIL - Center for Infection and Immunity of Lille, F-59000 Lille, France., Gonzalez-Rodriguez D; Université de Lorraine, LCP-A2MC, F-57000 Metz, France., Morel C; Institut Pasteur, Université de Paris, CNRS UMR6047, Inserm U1306, Unité des Toxines Bactériennes, 75015 Paris, France., Mettouchi A; Institut Pasteur, Université de Paris, CNRS UMR6047, Inserm U1306, Unité des Toxines Bactériennes, 75015 Paris, France., Debayle D; Institut de Pharmacologie Moléculaire et Cellulaire, UMR 7275, CNRS and Université Côte d'Azur, 06560 Valbonne, France., Dallongeville S; Institut Pasteur, BioImage Analysis Unit, CNRS UMR 3691, Paris, France., Olivo-Marin JC; Institut Pasteur, BioImage Analysis Unit, CNRS UMR 3691, Paris, France., Antonny B; Institut de Pharmacologie Moléculaire et Cellulaire, UMR 7275, CNRS and Université Côte d'Azur, 06560 Valbonne, France., Lafont F; Université de Lille, CNRS, Inserm, CHU Lille, Institut Pasteur Lille, U1019 - UMR 9017 - CIIL - Center for Infection and Immunity of Lille, F-59000 Lille, France., Lemichez E; Institut Pasteur, Université de Paris, CNRS UMR6047, Inserm U1306, Unité des Toxines Bactériennes, 75015 Paris, France., Barelli H; Institut de Pharmacologie Moléculaire et Cellulaire, UMR 7275, CNRS and Université Côte d'Azur, 06560 Valbonne, France.
Jazyk:	angličtina
Zdroj:	Journal of cell science [J Cell Sci] 2022 Mar 01; Vol. 135 (5). Date of Electronic Publication: 2022 Feb 08.
DOI:	10.1242/jcs.259119
Abstrakt:	Metabolic studies and animal knockout models point to the critical role of polyunsaturated docosahexaenoic acid (22:6, DHA)-containing phospholipids (DHA-PLs) in physiology. Here, we investigated the impact of DHA-PLs on the dynamics of transendothelial cell macroapertures (TEMs) triggered by RhoA inhibition-associated cell spreading. Lipidomic analyses showed that human umbilical vein endothelial cells (HUVECs) subjected to a DHA diet undergo a 6-fold enrichment in DHA-PLs at the plasma membrane (PM) at the expense of monounsaturated oleic acid-containing PLs (OA-PLs). Consequently, DHA-PL enrichment at the PM induces a reduction in cell thickness and shifts cellular membranes towards a permissive mode of membrane fusion for transcellular tunnel initiation. We provide evidence that a global homeostatic control of membrane tension and cell cortex rigidity minimizes overall changes of TEM area through a decrease of TEM size and lifetime. Conversely, low DHA-PL levels at the PM lead to the opening of unstable and wider TEMs. Together, this provides evidence that variations of DHA-PL levels in membranes affect cell biomechanical properties. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2022. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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