A population based expression atlas provides insights into disease resistance and other physiological traits in cassava (Manihot esculenta Crantz).

Autor: Ogbonna AC; Section on Plant Breeding and Genetics, School of Integrative Plant Sciences, Cornell University, Ithaca, 14850, USA. aco46@cornell.edu.; Boyce Thompson Institute, 533 Tower Rd, Ithaca, 14850, USA. aco46@cornell.edu., Ramu P; Section on Plant Breeding and Genetics, School of Integrative Plant Sciences, Cornell University, Ithaca, 14850, USA., Esuma W; National Crops Resources Research Institute, Namulonge, P.O.Box 7084, Uganda, Uganda., Nandudu L; Section on Plant Breeding and Genetics, School of Integrative Plant Sciences, Cornell University, Ithaca, 14850, USA.; National Crops Resources Research Institute, Namulonge, P.O.Box 7084, Uganda, Uganda., Morales N; Section on Plant Breeding and Genetics, School of Integrative Plant Sciences, Cornell University, Ithaca, 14850, USA., Powell A; Boyce Thompson Institute, 533 Tower Rd, Ithaca, 14850, USA., Kawuki R; National Crops Resources Research Institute, Namulonge, P.O.Box 7084, Uganda, Uganda., Bauchet G; Boyce Thompson Institute, 533 Tower Rd, Ithaca, 14850, USA., Jannink JL; United States Department of Agriculture US, Washington, USA., Mueller LA; Section on Plant Breeding and Genetics, School of Integrative Plant Sciences, Cornell University, Ithaca, 14850, USA. lam87@cornell.edu.; Boyce Thompson Institute, 533 Tower Rd, Ithaca, 14850, USA. lam87@cornell.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Dec 07; Vol. 11 (1), pp. 23520. Date of Electronic Publication: 2021 Dec 07.
DOI: 10.1038/s41598-021-02794-y
Abstrakt: Cassava, a food security crop in Africa, is grown throughout the tropics and subtropics. Although cassava can provide high productivity in suboptimal conditions, the yield in Africa is substantially lower than in other geographies. The yield gap is attributable to many challenges faced by cassava in Africa, including susceptibility to diseases and poor soil conditions. In this study, we carried out 3'RNA sequencing on 150 accessions from the National Crops Resources Research Institute, Uganda for 5 tissue types, providing population-based transcriptomics resources to the research community in a web-based queryable cassava expression atlas. Differential expression and weighted gene co-expression network analysis were performed to detect 8820 significantly differentially expressed genes (DEGs), revealing similarity in expression patterns between tissue types and the clustering of detected DEGs into 18 gene modules. As a confirmation of data quality, differential expression and pathway analysis targeting cassava mosaic disease (CMD) identified 27 genes observed in the plant-pathogen interaction pathway, several previously identified CMD resistance genes, and two peroxidase family proteins different from the CMD2 gene. Present research work represents a novel resource towards understanding complex traits at expression and molecular levels for the development of resistant and high-yielding cassava varieties, as exemplified with CMD.
(© 2021. The Author(s).)
Databáze: MEDLINE
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