Cell Aggregation Assays for Homophilic Interactions Between Cell Surface Proteins.
| Autor: | Huene AL; Department of Surgery and Center for Evolutionary Biology and Medicine, University of Pittsburgh, Pittsburgh, PA, USA., Nicotra ML; Departments of Surgery and Immunology, Thomas E. Starzl Transplantation Institute, and Center for Evolutionary Biology and Medicine, University of Pittsburgh, Pittsburgh, PA, USA. matthew.nicotra@pitt.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2421, pp. 91-102. |
| DOI: | 10.1007/978-1-0716-1944-5_6 |
| Abstrakt: | Many proteins expressed on the cellular surface provide signaling and cell adhesion properties required for vital cellular functions. These binding interactions can occur between different but complementary proteins such as a ligand and receptor, or between the same protein acting as both ligand and receptor. The cell aggregation assay is a straightforward technique to identify homophilic interactions from such proteins. Here we describe the procedure for testing proteins via cell aggregation assays in HEK293T cells. (© 2022. Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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