Complement-Mediated Differential Immune Response of Human Macrophages to Sporothrix Species Through Interaction With Their Cell Wall Peptidorhamnomannans.

Autor:	Neves GWP; Cell Biology Department, Rio de Janeiro State University, Rio de Janeiro, Brazil., Wong SSW; Institut Pasteur, Molecular Mycology Unit, CNRS UMR2000, Paris, France., Aimanianda V; Institut Pasteur, Molecular Mycology Unit, CNRS UMR2000, Paris, France., Simenel C; Institut Pasteur, Biological NMR and HDX-MS Technological Platform, CNRS UMR3528, Paris, France., Guijarro JI; Institut Pasteur, Biological NMR and HDX-MS Technological Platform, CNRS UMR3528, Paris, France., Walls C; Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom., Willment JA; Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom.; Medical Research Council Centre for Medical Mycology at the University of Exeter, Exeter, United Kingdom., Gow NAR; Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom.; Medical Research Council Centre for Medical Mycology at the University of Exeter, Exeter, United Kingdom., Munro CA; Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom., Brown GD; Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom.; Medical Research Council Centre for Medical Mycology at the University of Exeter, Exeter, United Kingdom., Lopes-Bezerra LM; Cell Biology Department, Rio de Janeiro State University, Rio de Janeiro, Brazil.; Biomedical Institute and Technology and Innovation Center (CIETEC), São Paulo University, São Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2021 Nov 15; Vol. 12, pp. 749074. Date of Electronic Publication: 2021 Nov 15 (Print Publication: 2021).
DOI:	10.3389/fimmu.2021.749074
Abstrakt:	In this study, the human immune response mechanisms against Sporothrix brasiliensis and Sporothrix schenckii , two causative agents of human and animal sporotrichosis, were investigated. The interaction of S. brasiliensis and S. schenckii with human monocyte-derived macrophages (hMDMs) was shown to be dependent on the thermolabile serum complement protein C3, which facilitated the phagocytosis of Sporothrix yeast cells through opsonization. The peptidorhamnomannan (PRM) component of the cell walls of these two Sporothrix yeasts was found to be one of their surfaces exposed pathogen-associated molecular pattern (PAMP), leading to activation of the complement system and deposition of C3b on the Sporothrix yeast surfaces. PRM also showed direct interaction with CD11b, the specific component of the complement receptor-3 (CR3). Furthermore, the blockade of CR3 specifically impacted the interleukin (IL)-1β secretion by hMDM in response to both S. brasiliensis and S. schenckii , suggesting that the host complement system plays an essential role in the inflammatory immune response against these Sporothrix species. Nevertheless, the structural differences in the PRMs of the two Sporothrix species, as revealed by NMR, were related to the differences observed in the host complement activation pathways. Together, this work reports a new PAMP of the cell surface of pathogenic fungi playing a role through the activation of complement system and via CR3 receptor mediating an inflammatory response to Sporothrix species. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Neves, Wong, Aimanianda, Simenel, Guijarro, Walls, Willment, Gow, Munro, Brown and Lopes-Bezerra.)
Databáze:	MEDLINE
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