Measurement of fish freshness: Flow cytometry analysis of isolated muscle mitochondria.

Autor: Soret M; Univ. Littoral Côte d'Opale, UMR 1158 BioEcoAgro, Institut Charles Viollette, USC ANSES, INRAE, Univ. Artois, Univ. Lille, Univ. Picardie Jules Verne, Univ. Liège, Junia, F-62200 Boulogne-sur-Mer, France. Electronic address: meline.soret@univ-littoral.fr., Bouchendhomme T; Univ. Littoral Côte d'Opale, UMR 1158 BioEcoAgro, Institut Charles Viollette, USC ANSES, INRAE, Univ. Artois, Univ. Lille, Univ. Picardie Jules Verne, Univ. Liège, Junia, F-62200 Boulogne-sur-Mer, France. Electronic address: tiffanie.bouchendhomme@univ-littoral.fr., Cleach J; Univ. Littoral Côte d'Opale, UMR 1158 BioEcoAgro, Institut Charles Viollette, USC ANSES, INRAE, Univ. Artois, Univ. Lille, Univ. Picardie Jules Verne, Univ. Liège, Junia, F-62200 Boulogne-sur-Mer, France. Electronic address: jerome.cleach@univ-littoral.fr., Jouy N; Univ. Lille, Bio Imaging Center Lille, Lille, F-59000, France. Electronic address: nathalie.jouy@inserm.fr., Crola Da Silva C; Univ. Claude Bernard Lyon 1, CarMeN Laboratory, INSERM U1060, INRAe, INSA, F-69500 Bron, France. Electronic address: claire.crola-da-silva@univ-lyon1.fr., Devin A; UMR CNRS 5095 Institut de biochimie et génétique cellulaires (IBGC), F-33077 Bordeaux, France. Electronic address: anne.devin@ibgc.cnrs.fr., Grard T; Univ. Littoral Côte d'Opale, UMR 1158 BioEcoAgro, Institut Charles Viollette, USC ANSES, INRAE, Univ. Artois, Univ. Lille, Univ. Picardie Jules Verne, Univ. Liège, Junia, F-62200 Boulogne-sur-Mer, France. Electronic address: thierry.grard@univ-littoral.fr., Lencel P; Univ. Littoral Côte d'Opale, UMR 1158 BioEcoAgro, Institut Charles Viollette, USC ANSES, INRAE, Univ. Artois, Univ. Lille, Univ. Picardie Jules Verne, Univ. Liège, Junia, F-62200 Boulogne-sur-Mer, France. Electronic address: philippe.lencel@univ-littoral.fr.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2022 Mar 30; Vol. 373 (Pt B), pp. 131690. Date of Electronic Publication: 2021 Nov 27.
DOI: 10.1016/j.foodchem.2021.131690
Abstrakt: Mitochondria are real sensors of the physiological status of tissues. After the death of an animal, they maintain physiological activity for several days. This activity is highly dependent on the availability of nutrients in the tissue. In this study, flow cytometry was used to measure the membrane potential of mitochondria isolated from European seabass (Dicentrarchus labrax) red muscle stored in ice for seven days in order to characterize fish freshness. Two probes, TMRM and Rhodamine 123, were used to measure mitochondrial potential. During the first few days (D0 to D3), isolated mitochondria maintained high potential, and then lost their potential (from D3 to D5), but were always re-polarizable after addition of substrates (glutamate, malate and succinate). From D7, the mitochondria were more strongly depolarized and were difficult to repolarize by the substrates. Using flow cytometry, we demonstrated that mitochondria were an excellent marker to confirm seabass freshness.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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