Three-dimensional liquid chromatography with parallel second dimensions and quadruple parallel mass spectrometry for adult/infant formula analysis.

Autor: Byrdwell WC; Methods and Application of Food Composition Lab, Agricultural Research Service, U.S. Dept. of Agriculture, 10300 Baltimore Ave., Beltsville, MD, 20705, USA. Electronic address: Craig.Byrdwell@usda.gov., Kotapati HK; Methods and Application of Food Composition Lab, Agricultural Research Service, U.S. Dept. of Agriculture, 10300 Baltimore Ave., Beltsville, MD, 20705, USA., Goldschmidt R; Methods and Application of Food Composition Lab, Agricultural Research Service, U.S. Dept. of Agriculture, 10300 Baltimore Ave., Beltsville, MD, 20705, USA., Jakubec P; Charles University, Faculty of Pharmacy, Dept. of Analytical Chemistry, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic., Nováková L; Charles University, Faculty of Pharmacy, Dept. of Analytical Chemistry, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
Jazyk: angličtina
Zdroj: Journal of chromatography. A [J Chromatogr A] 2022 Jan 04; Vol. 1661, pp. 462682. Date of Electronic Publication: 2021 Nov 22.
DOI: 10.1016/j.chroma.2021.462682
Abstrakt: Three dimensions of chromatographic separation, using split-flow two-dimensional liquid chromatography (SF-2D-LC) with two parallel second dimensions, LC × 2LC, combined with quadruple parallel mass spectrometry (LC3MS4) is demonstrated for analysis of NIST SRM 1849a adult/infant formula. The first dimension, 1 D, was a conventional non-aqueous reversed-phase (NARP) HPLC separation using two C18 columns in series, followed by detection using an ultraviolet (UV) detector, a fluorescence detector (FLD), with flow then split to a corona charged aerosol detector (CAD), and then dual parallel mass spectrometry (MS), conducted in atmospheric pressure photoionization (APPI) and electrospray ionization (ESI) modes. The first second dimension, 2 D(1), UHPLC was conducted on a 50.0 mm C30 column using a NARP-UHPLC parallel gradient for separation of short-chain triacylglycerols (TAGs) from long-chain TAGs, with detection by UV and ESI-MS. The second dimension, 2 D(2), UHPLC was conducted using a 100.0 mm C30 column with a NARP-UHPLC parallel gradient for improved separation of TAG isomers, with detection by UV, an evaporative light scattering detector, and high-resolution, accurate-mass (HRAM) ESI-MS. Transferred eluent dilution was used to refocus peaks and keep them sharp during elution in both 2 Ds. The separation space in the 2 D(2) was optimized using multi-cycle (aka, "constructive wraparound") elution, which employed flow rate programming. In the 1 D, calibration lines for quantification of fat-soluble vitamins were constructed. A lipidomics approach to TAG identification and quantification by HRAM-ESI-MS was applied to the 2 D(2). These experiments can be represented: LC1MS2 × (LC1MS1 + LC1MS1) = LC3MS4, or three-dimensional liquid chromatography with quadruple parallel mass spectrometry.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Published by Elsevier B.V.)
Databáze: MEDLINE
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