Modulation of plant plasma membrane structure by exogenous fatty acid hydroperoxide is a potential perception mechanism for their eliciting activity.

Autor: Deboever E; Laboratory of Molecular Biophysics at Interfaces, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium.; Laboratory of Natural Molecules Chemistry, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium.; FytoFend S.A., Isnes, Belgium., Van Aubel G; FytoFend S.A., Isnes, Belgium.; Research Unit in Plant Cellular and Molecular Biology, University of Namur, Namur, Belgium., Rondelli V; Department of Medical Biotechnologies and Translational Medicine, Università degli Studi di Milano, Segrate, Italy., Koutsioubas A; Jülich Centre for Neutron Science (JCNS) at Heinz Maier-Leibnitz Zentrum (MLZ), Forschungszentrum Jülich GmbH, Garching, Germany., Mathelie-Guinlet M; Institute of Biomolecular Science and Technology (IBST), Louvain-la-Neuve, Belgium., Dufrene YF; Institute of Biomolecular Science and Technology (IBST), Louvain-la-Neuve, Belgium., Ongena M; Microbial Processes and Interactions (MiPI), Gembloux Agro-Bio Tech, Université de Liège, Gembloux, Belgium., Lins L; Laboratory of Molecular Biophysics at Interfaces, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium., Van Cutsem P; FytoFend S.A., Isnes, Belgium.; Research Unit in Plant Cellular and Molecular Biology, University of Namur, Namur, Belgium., Fauconnier ML; Laboratory of Natural Molecules Chemistry, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium., Deleu M; Laboratory of Molecular Biophysics at Interfaces, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium.
Jazyk: angličtina
Zdroj: Plant, cell & environment [Plant Cell Environ] 2022 Apr; Vol. 45 (4), pp. 1082-1095. Date of Electronic Publication: 2022 Jan 12.
DOI: 10.1111/pce.14239
Abstrakt: Oxylipins are lipid-derived molecules that are ubiquitous in eukaryotes and whose functions in plant physiology have been widely reported. They appear to play a major role in plant immunity by orchestrating reactive oxygen species (ROS) and hormone-dependent signalling pathways. The present work focuses on the specific case of fatty acid hydroperoxides (HPOs). Although some studies report their potential use as exogenous biocontrol agents for plant protection, evaluation of their efficiency in planta is lacking and no information is available about their mechanism of action. In this study, the potential of 13(S)-hydroperoxy-(9Z, 11E)-octadecadienoic acid (13-HPOD) and 13(S)-hydroperoxy-(9Z, 11E, 15Z)-octadecatrienoic acid (13-HPOT), as plant defence elicitors and the underlying mechanism of action is investigated. Arabidopsis thaliana leaf resistance to Botrytis cinerea was observed after root application with HPOs. They also activate early immunity-related defence responses, like ROS. As previous studies have demonstrated their ability to interact with plant plasma membranes (PPM), we have further investigated the effects of HPOs on biomimetic PPM structure using complementary biophysics tools. Results show that HPO insertion into PPM impacts its global structure without solubilizing it. The relationship between biological assays and biophysical analysis suggests that lipid amphiphilic elicitors that directly act on membrane lipids might trigger early plant defence events.
(© 2021 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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