Mesenchymal Stem Cells Enhance Chemotaxis of Activated T Cells through the CCL2-CCR2 Axis In Vitro.

Autor: Zhang YL; Department of General Practice, the Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China., Qiao SK; Department of Hematology, the Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China. saidesa@163.com., Xing LN; Department of Hematology, the Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China., Guo XN; Department of Hematology, the Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China., Ren JH; Department of Hematology, the Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2021 Dec; Vol. 172 (2), pp. 263-269. Date of Electronic Publication: 2021 Dec 02.
DOI: 10.1007/s10517-021-05373-3
Abstrakt: Activation and migration of donor T cells to the host target organs are critical mechanisms in the pathogenesis of graft-versus-host disease (GVHD). The role of monocyte chemoattractant protein-1 (MCP-1/CCL2) and its receptor CCR2 in the recruitment of T cells during immune or inflammatory response is also well known. For elucidation of the mechanism of the therapeutic effect of human bone marrow derived-mesenchymal stem cells (MSC) in GVHD, we studied the effect of these cells on migration of activated donor T cells through the CCL2-CCR2 axis in vitro. MSC were expanded from donors' bone marrow mononuclear cells. After co-culturing of IL-2-activated T cells with allogeneic MSC at different ratios, the levels of CCL2 in supernatants were measured by ELISA, and CCR2 expression in CD4 + /CD8 + T cells subsets were detected by flow cytometry. The effect of MSC on the migration of activated T cells in the Transwell system was studied in the absence or presence of CCL2. Our results show that CCL2 levels in supernatants of co-cultures were significantly higher than in MSC monoculture and this increase depended on the number of MSC. MSC inhibited proliferation of T cells, but did not change the percentages of CD4 + and CD8 + T cells subsets. MSC can up-regulate the CCR2 expression in CD8 + subsets rather than in CD4 + subsets; MSC enhanced migration of IL-2-activated T cells to CCL2 by increasing the expression of CCR2. The data demonstrate that MSC can enhance chemotaxis of cytokine-activated T cells through the CCL2-CCR2 axis in vitro.
(© 2021. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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