Improved Survival and Regeneration of Irradiated Mouse Neural Stem Cells after Co-Culturing with Non-Irradiated Mouse Neural Stem Cells or Mesenchymal Stem Cells from the Adipose Tissue.

Autor: Ratushnyak MG; National Research Center 'Kurchatov Institute', Moscow, Russia. ratushnyak_marya@mail.ru., Semochkina YP; National Research Center 'Kurchatov Institute', Moscow, Russia., Zhirnik AS; National Research Center 'Kurchatov Institute', Moscow, Russia., Smirnova OD; National Research Center 'Kurchatov Institute', Moscow, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2021 Dec; Vol. 172 (2), pp. 228-235. Date of Electronic Publication: 2021 Dec 02.
DOI: 10.1007/s10517-021-05368-0
Abstrakt: We studied the effect of neural stem cells (NSC) and mesenchymal stem cells (MSC) from mouse adipose tissue on survival, clonogenic activity, and senescence of NSC after exposure to γ-radiation. It was found that survival and clonogenic activity of NSC irradiated in doses of 1 and 2 Gy was enhanced when irradiated cells were co-cultured with non-irradiated NSC and MSC in permeable Transwell inserts. The proportion of senescent NSC (cells with high β-galactosidase activity) increased with increasing irradiation dose. Co-culturing with non-irradiated NSC in 3 days after irradiation in a dose of 1 Gy led to a decrease in the proportion of senescent cells among irradiated NSC. Factors secreted by NSC and MSC can become the basis for the development of means for prevention and treatment of damage to brain cells resulting from radiation therapy of head and neck cancer.
(© 2021. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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