Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis.

Autor: Kinoshita Y; Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan., Kakoi H; Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan., Ishige T; Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan., Yamanaka T; Epizootic Prevention Section, Equine Department, Japan Racing Association, 1-1-1 Nishi Shinnbashi, Minato-ku, Tokyo 105-0003, Japan., Niwa H; Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan., Uchida-Fujii E; Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan., Nukada T; Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan., Ueno T; Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
Jazyk: angličtina
Zdroj: The Journal of veterinary medical science [J Vet Med Sci] 2022 Jan 24; Vol. 84 (1), pp. 129-132. Date of Electronic Publication: 2021 Dec 02.
DOI: 10.1292/jvms.21-0539
Abstrakt: Taylorella equigenitalis causes contagious equine metritis. Here we compared seven nucleic acid amplification tests for T. equigenitalis to select a rapid and reliable diagnostic method. The 95% detection limits of each assay varied greatly: real-time PCR had the lowest detection limit (0.77 fg/reaction); those of some of the conventional PCRs (cPCRs) were >100 fg/reaction. In experimentally infected samples, real-time PCR and semi-nested PCR showed the highest positive numbers (33 out of 42 samples), but two of the cPCRs detected only 2 and 7 positive results. Our results indicate that the use of sensitive molecular assays is important for the efficient detection of T. equigenitalis in clinical samples.
Databáze: MEDLINE
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