The use of ex-vivo liver perfusion circuit in sheep model: Preliminary report.

Autor:	Al-Sebayel MI; From the Department of Liver Transplantation & Hepatobiliary-Pancreatic Surgery (Al-Sebayel, El-Sheikh, Al Abbad, Al Nemry, Al-Jammali, Al-Zeer, Alsebayel, Al-Bahli); and from the Department of Comparative Medicine (Al-Mohanna), King Faisal Specialist Hospital & Research Center, Riyadh, Kingdom of Saudi Arabia., El-Sheikh YM; From the Department of Liver Transplantation & Hepatobiliary-Pancreatic Surgery (Al-Sebayel, El-Sheikh, Al Abbad, Al Nemry, Al-Jammali, Al-Zeer, Alsebayel, Al-Bahli); and from the Department of Comparative Medicine (Al-Mohanna), King Faisal Specialist Hospital & Research Center, Riyadh, Kingdom of Saudi Arabia., Al-Mohanna FH; From the Department of Liver Transplantation & Hepatobiliary-Pancreatic Surgery (Al-Sebayel, El-Sheikh, Al Abbad, Al Nemry, Al-Jammali, Al-Zeer, Alsebayel, Al-Bahli); and from the Department of Comparative Medicine (Al-Mohanna), King Faisal Specialist Hospital & Research Center, Riyadh, Kingdom of Saudi Arabia., Al Abbad SI; From the Department of Liver Transplantation & Hepatobiliary-Pancreatic Surgery (Al-Sebayel, El-Sheikh, Al Abbad, Al Nemry, Al-Jammali, Al-Zeer, Alsebayel, Al-Bahli); and from the Department of Comparative Medicine (Al-Mohanna), King Faisal Specialist Hospital & Research Center, Riyadh, Kingdom of Saudi Arabia., Al Nemry YH; From the Department of Liver Transplantation & Hepatobiliary-Pancreatic Surgery (Al-Sebayel, El-Sheikh, Al Abbad, Al Nemry, Al-Jammali, Al-Zeer, Alsebayel, Al-Bahli); and from the Department of Comparative Medicine (Al-Mohanna), King Faisal Specialist Hospital & Research Center, Riyadh, Kingdom of Saudi Arabia., Al-Jammali A; From the Department of Liver Transplantation & Hepatobiliary-Pancreatic Surgery (Al-Sebayel, El-Sheikh, Al Abbad, Al Nemry, Al-Jammali, Al-Zeer, Alsebayel, Al-Bahli); and from the Department of Comparative Medicine (Al-Mohanna), King Faisal Specialist Hospital & Research Center, Riyadh, Kingdom of Saudi Arabia., Al-Zeer NK; From the Department of Liver Transplantation & Hepatobiliary-Pancreatic Surgery (Al-Sebayel, El-Sheikh, Al Abbad, Al Nemry, Al-Jammali, Al-Zeer, Alsebayel, Al-Bahli); and from the Department of Comparative Medicine (Al-Mohanna), King Faisal Specialist Hospital & Research Center, Riyadh, Kingdom of Saudi Arabia., Alsebayel YM; From the Department of Liver Transplantation & Hepatobiliary-Pancreatic Surgery (Al-Sebayel, El-Sheikh, Al Abbad, Al Nemry, Al-Jammali, Al-Zeer, Alsebayel, Al-Bahli); and from the Department of Comparative Medicine (Al-Mohanna), King Faisal Specialist Hospital & Research Center, Riyadh, Kingdom of Saudi Arabia., Al-Bahli HM; From the Department of Liver Transplantation & Hepatobiliary-Pancreatic Surgery (Al-Sebayel, El-Sheikh, Al Abbad, Al Nemry, Al-Jammali, Al-Zeer, Alsebayel, Al-Bahli); and from the Department of Comparative Medicine (Al-Mohanna), King Faisal Specialist Hospital & Research Center, Riyadh, Kingdom of Saudi Arabia.
Jazyk:	angličtina
Zdroj:	Saudi medical journal [Saudi Med J] 2021 Dec; Vol. 42 (12), pp. 1289-1295.
DOI:	10.15537/smj.2021.42.12.20210054
Abstrakt:	Objectives: To describe a novel animal model for ex-vivo liver perfusion. Methods: This study was carried out at King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia, between September 2016 and January 2019. We assembled a perfusion circuit operated by a continuous pressure-driven arterial pump with continuous portal and arterial pressure and volume measurements. We used normothermic oxygenated perfusate. The livers used were retrieved from the sheep. Results: Ex-vivo continuous perfusion of the liver was achieved for up to 9 hours with stable pressure and volume in both hepatic artery and portal vein. In 4 experiments the arterial pressure was kept in a range of 48-52 mmHg with a mean of 51.75±4.31 resulting in arterial volume at steady state of 223.5±48.25 ml/minute (95% confidence level). At steady state the mean portal pressure was 16.25±1.45 mmHg with a mean volume of 854±313.75 ml/minute (95% confidence level). Bile production was observed during the perfusion period. Hemodynamic parameters were similar to the physiological parameters observed in normothermic perfusion model of the porcine liver. Conclusion: A normothermic oxygenated ex-vivo perfusion circuit was successfully constructed using the sheep liver. A sustainable functional circuit with physiological hemodynamic parameters was achieved. Further study on sheep model seems to be feasible. (Copyright: © Saudi Medical Journal.)
Databáze:	MEDLINE
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