Opto-vTrap, an optogenetic trap for reversible inhibition of vesicular release, synaptic transmission, and behavior.

Autor:	Won J; Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon 34126, Republic of Korea; Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea., Pankratov Y; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK., Jang MW; Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon 34126, Republic of Korea; KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 02841, Republic of Korea., Kim S; Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon 34126, Republic of Korea; KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 02841, Republic of Korea., Ju YH; Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon 34126, Republic of Korea., Lee S; Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon 34126, Republic of Korea., Lee SE; Virus Facility, Research Animal Resource Center, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea., Kim A; Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon 34126, Republic of Korea., Park S; Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon 34126, Republic of Korea., Lee CJ; Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon 34126, Republic of Korea; KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 02841, Republic of Korea. Electronic address: cjl@ibs.re.kr., Heo WD; Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon 34126, Republic of Korea; Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea; KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea. Electronic address: wondo@kaist.ac.kr.
Jazyk:	angličtina
Zdroj:	Neuron [Neuron] 2022 Feb 02; Vol. 110 (3), pp. 423-435.e4. Date of Electronic Publication: 2021 Nov 30.
DOI:	10.1016/j.neuron.2021.11.003
Abstrakt:	Spatiotemporal control of brain activity by optogenetics has emerged as an essential tool to study brain function. For silencing brain activity, optogenetic probes, such as halorhodopsin and archaerhodopsin, inhibit transmitter release indirectly by hyperpolarizing membrane potentials. However, these probes cause an undesirable ionic imbalance and rebound spikes. Moreover, they are not applicable to use in non-excitable glial cells. Here we engineered Opto-vTrap, a light-inducible and reversible inhibition system to temporarily trap the transmitter-containing vesicles from exocytotic release. Light activation of Opto-vTrap caused full vesicle clusterization and complete inhibition of exocytosis within 1 min, which recovered within 30 min after light off. We found a significant reduction in synaptic and gliotransmission upon activation of Opto-vTrap in acute brain slices. Opto-vTrap significantly inhibited hippocampus-dependent memory retrieval with full recovery within an hour. We propose Opto-vTrap as a next-generation optogenetic silencer to control brain activity and behavior with minimal confounding effects. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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