Changes in Fecal Pellet Microbiome of the Cold-Adapted Antarctic Copepod Tigriopus kingsejongensis at Different Temperatures and Developmental Stages.

Autor: Oh HN; Department of Systems Biotechnology, Chung-Ang University, Anseong, 17546, Republic of Korea., Myeong NR; Department of Systems Biotechnology, Chung-Ang University, Anseong, 17546, Republic of Korea.; Division of Life Sciences, Korea Polar Research Institute, Incheon, 21990, Republic of Korea., Kim T; Department of Systems Biotechnology, Chung-Ang University, Anseong, 17546, Republic of Korea., Min GS; Department of Biological Sciences, Inha University, Incheon, 22212, Republic of Korea., Kim S; Division of Life Sciences, Korea Polar Research Institute, Incheon, 21990, Republic of Korea., Sul WJ; Department of Systems Biotechnology, Chung-Ang University, Anseong, 17546, Republic of Korea. sulwj@cau.ac.kr.
Jazyk: angličtina
Zdroj: Microbial ecology [Microb Ecol] 2022 Nov; Vol. 84 (4), pp. 1029-1041. Date of Electronic Publication: 2021 Dec 01.
DOI: 10.1007/s00248-021-01928-z
Abstrakt: Tigriopus kingsejongensis, a copepod species reported from the King Sejong Station, Antarctica, serves as a valuable food resource in ecosystems. We cultured T. kingsejongensis at three different temperatures (2 °C, 8 °C, and 15 °C) in a laboratory to observe the changes in its fecal pellet microbiome depending on the cultivation temperatures and developmental stages. We observed that the fecal pellet microbiome of the copepod changed with temperature: a lower microbial diversity, higher abundance of the aquatic bacterium Vibrio, and lower abundance of the psychrophilic bacterium Colwellia were noted at higher temperatures. In addition, the fecal pellet microbiome of the copepod changed according to the developmental stage: a lower microbial diversity was noted in egg-attached copepods than in nauplii at 8 °C. We further analyzed three shotgun metagenomes from the fecal pellet samples of T. kingsejongensis at different temperatures and obtained 44 metagenome-assembled genomes (MAGs). We noted that MAGs of V. splendidus D contained glycosyl hydrolases (GHs) encoding chitinases and virulence factors at a higher relative abundance at 15 °C than at lower temperatures. These results indicate that increasing temperature affects the fecal pellet microbiome and the development of copepods. The findings are helpful to understand the changes in cold-adapted copepods and the effect of temperature on their growth.
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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