The genetic basis of differential autodiploidization in evolving yeast populations.

Autor: Tung S; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA.; The Lakshmi Mittal And Family South Asia Institute, Harvard University, Cambridge, MA 02138, USA., Bakerlee CW; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA.; Quantitative Biology Initiative, Harvard University, Cambridge, MA 02138, USA.; NSF-Simons Center for Mathematical and Statistical Analysis of Biology, Harvard University, Cambridge, MA 02138, USA.; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA., Phillips AM; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA., Nguyen Ba AN; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA.; Quantitative Biology Initiative, Harvard University, Cambridge, MA 02138, USA.; NSF-Simons Center for Mathematical and Statistical Analysis of Biology, Harvard University, Cambridge, MA 02138, USA., Desai MM; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA.; Quantitative Biology Initiative, Harvard University, Cambridge, MA 02138, USA.; NSF-Simons Center for Mathematical and Statistical Analysis of Biology, Harvard University, Cambridge, MA 02138, USA.; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.; Department of Physics, Harvard University, Cambridge, MA 02138, USA.
Jazyk: angličtina
Zdroj: G3 (Bethesda, Md.) [G3 (Bethesda)] 2021 Aug 07; Vol. 11 (8).
DOI: 10.1093/g3journal/jkab192
Abstrakt: Spontaneous whole-genome duplication, or autodiploidization, is a common route to adaptation in experimental evolution of haploid budding yeast populations. The rate at which autodiploids fix in these populations appears to vary across strain backgrounds, but the genetic basis of these differences remains poorly characterized. Here, we show that the frequency of autodiploidization differs dramatically between two closely related laboratory strains of Saccharomyces cerevisiae, BY4741 and W303. To investigate the genetic basis of this difference, we crossed these strains to generate hundreds of unique F1 segregants and tested the tendency of each segregant to autodiplodize across hundreds of generations of laboratory evolution. We find that variants in the SSD1 gene are the primary genetic determinant of differences in autodiploidization. We then used multiple laboratory and wild strains of S. cerevisiae to show that clonal populations of strains with a functional copy of SSD1 autodiploidize more frequently in evolution experiments, while knocking out this gene or replacing it with the W303 allele reduces autodiploidization propensity across all genetic backgrounds tested. These results suggest a potential strategy for modifying rates of spontaneous whole-genome duplications in laboratory evolution experiments in haploid budding yeast. They may also have relevance to other settings in which eukaryotic genome stability plays an important role, such as biomanufacturing and the treatment of pathogenic fungal diseases and cancers.
(© The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.)
Databáze: MEDLINE
Externí odkaz: