Activity of Free and Liposomal Antimony Trioxide in the Acute Promyelocytic Leukemia Cell Line NB4.

Autor:	Viana AR; Área de Ciências da Saúde e Tecnológicas, Universidade Franciscana (UFN), Santa Maria, RS, Brasil., Davidson CB; Área de Ciências da Saúde e Tecnológicas, Universidade Franciscana (UFN), Santa Maria, RS, Brasil., Salles B; Área de Ciências da Saúde e Tecnológicas, Universidade Franciscana (UFN), Santa Maria, RS, Brasil., DE Almeida LY; Departamento de Clínica Médica, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo (FMRP-USP), Ribeirão Preto, SP, Brasil., Krause A; Departamento de Clínica de Pequenos Animais, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brasil., Bizzi CA; Departamento de Química, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brasil., Rego EM; Departamento de Clínica Médica, Faculdade de Medicina, Universidade de São Paulo (FM-USP), São Paulo, SP, Brasil., Fontanari Krause LM; Departamento de Morfologia, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brasil., Mortari SR; Área de Ciências da Saúde e Tecnológicas, Universidade Franciscana (UFN), Santa Maria, RS, Brasil; mortari@ufn.edu.br.
Jazyk:	angličtina
Zdroj:	Anticancer research [Anticancer Res] 2021 Dec; Vol. 41 (12), pp. 6061-6065.
DOI:	10.21873/anticanres.15425
Abstrakt:	Background/aim: Antimony is a chemical element used in the therapy of parasitic diseases with a promising anticancer potential. The aim of this study was to evaluate in vitro activity of free or liposomal vesicle-packed antimony trioxide (AT or LAT) in the t(15;17)(q22;q21) translocation-positive acute promyelocytic leukemia (APL) cell line NB4. Materials and Methods: Cytotoxicity was analysed with trypan blue exclusion, the MTT assay and neutral red exclusion assay; cell proliferation with PicoGreen ® ; and reactive oxygen species (ROS) production with DCFDA. Results: Liposomal particles did not change the pH of the cell culture medium and entered the cells. Both formulations resulted in a time- and concentration-dependent cytotoxicity and production of ROS. LAT showed higher toxicity at lower concentrations compared to AT. Conclusion: LAT may be used to decrease drug dosage and maintain high anti-tumoral effects on APL cells. (Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
Databáze:	MEDLINE
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