| Autor: |
Break MKB; Department of Pharmaceutical Chemistry, College of Pharmacy, University of Hail, Hail, Saudi Arabia., Younes KM; Department of Pharmaceutical Chemistry, College of Pharmacy, University of Hail, Hail, Saudi Arabia.; Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Cairo, Egypt., Elkahoui S; Department of Biology, College of Science, University of Hail, Hail, Saudi Arabia.; Laboratory of Bioactive Substances, Center of Biotechnology of Borj Cedria (CBBC), Hammam Lif, Tunisia., Unissa R; Department of Pharmaceutics, College of Pharmacy, University of Hail, Hail, Saudi Arabia., Alfahidat SA; Department of Pharmaceutical Chemistry, College of Pharmacy, University of Hail, Hail, Saudi Arabia., Alshawi KS; Department of Pharmaceutical Chemistry, College of Pharmacy, University of Hail, Hail, Saudi Arabia., Abouzied AS; Department of Pharmaceutical Chemistry, College of Pharmacy, University of Hail, Hail, Saudi Arabia.; National Organization for Drug Control & Research, Cairo, Egypt. |
| Abstrakt: |
This study was done to evaluate the anticancer potential of Achillea fragrantissima (Forssk.) Sch.Bip. leaves methanolic extract in detail for the first time, in addition to investigating its antimicrobial activity. The antimicrobial assay revealed that the extract exerted high activity against P. vulgaris (MIC = 156.25 µg/ml) and C. albicans (MIC = 625 µg/ml), while moderate activity was observed against other microbes. The extract was also screened against HepG2, A549, HCT116 and MCF7 cancer cells and was found to be active across all cells with highest selectivity and cytotoxic activity being observed for A549 cells (IC 50 = 1.21 µg/ml). Further mechanistic studies on A549 cells showed that the extract resulted in S-phase arrest and induced apoptosis via activation of caspase-3, p53 and Bax, in addition to downregulation of Bcl-2. HR-LCMS analysis indicated the presence of 3-hydroxycoumarin, quercetin 3,3'-dimethyl ether and skullcapflavone II which might be responsible for the extract's bioactivity. |
